IL-12、TGF-β1在CRSwNP组织中的表达水平及其临床意义

Expression levels and clinical significance of IL-12 and TGF-β_(1) in CRSwNP tissues

目的 研究白介素-12 (IL-12)、转化生长因子β_(1)(TGF-β_(1))在慢性鼻-鼻窦炎伴鼻息肉(CRSwNP)组织中的表达水平及其临床意义。方法 将2020年1月至2020年12月于陕西中医药大学附属医院就诊的58例CRSwNP患者纳入观察组,另选择同期于本院就诊的55例慢性鼻-鼻窦炎不伴鼻息肉(CRSsNP)患者纳入对照组。比较观察组息肉组织及对照组鼻黏膜组织中IL-12、TGF-β_(1)表达水平的差异,根据Lund-Mackay内镜评分结果将观察组患者分为轻度组和重度组,根据炎性细胞浸润比例不同将观察组患者分为嗜酸性粒细胞组和非嗜酸性粒细胞组,根据疾病复发情况将观察组患者分为复发组和未复发组,比较不同组患者的IL-12、TGF-β_(1)表达水平,采用Spearman等级相关分析IL-12、TGF-β_(1)表达与嗜酸性粒细胞浸润百分比的相关性。结果 观察组患者的IL-12、TGF-β_(1)表达水平分别为(42.93±10.52) pg/mL、(529.89±147.67) ng/mL,明显高于对照组的(33.11±9.87) pg/m L、(455.39±134.04) ng/mL,差异均具有统计学意义(P<0.05);轻度组患者的IL-12、TGF-β_(1)表达水平分别为(40.16±9.64) pg/mL、(515.78±65.95) ng/mL,明显低于重度组的(49.61±9.98) pg/mL、(563.92±57.84) ng/mL,差异均有统计学意义(P<0.05);嗜酸性粒细胞组患者的IL-12、TGF-β_(1)表达水平分别为(47.08±10.15) pg/mL、(556.32±74.58) ng/mL,明显高于非嗜酸性粒细胞组的(36.14±10.68) pg/mL、(486.64±81.07) ng/mL,差异均有统计学意义(P<0.05);复发组患者的IL-12、TGF-β_(1)表达水平分别为(48.78±8.35) pg/mL、(571.29±74.84) ng/mL,明显高于未复发组的(40.08±9.06) pg/mL、(509.72±88.65) ng/mL,差异均有统计学意义(P<0.05);经Spearman等级相关分析结果显示,IL-12、TGF-β_(1)表达水平均与嗜酸性粒细胞浸润百分比呈正相关(P<0.05)。结论 IL-12、TGF-β_(1)在CRSwNP患者鼻息肉组织中呈现高表达水平,疾病进展以及嗜酸性粒细胞浸润程度较严重的患者IL-12、TGF-β_(1)表达水平较高,术后疾病复发风险也较高。

Objective To study the expression levels and clinical significance of interleukin-12(IL-12) and transforming growth factor β_(1)(TGF-β_(1)) in chronic rhinosinusitis with nasal polyps(CRSwNP) tissues.Methods A total of 58 patients with CRSwNP treated in Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine were enrolled as the observation group between January 2020 and December 2020,while 55 patients with chronic rhinosinusitis without nasal polyps(CRSsNP) during the same period were enrolled as the control group.The differences in the expression levels of IL-12 and TGF-β_(1) in polyp tissues of observation group and nasal mucosa tissues of control group were compared.According to Lund-Mackay endoscopy scores,patients in the observation group were divided into mild group and severe group.According to different proportions of inflammatory cells infiltration,patients in the observation group were divided into eosinophil group and non-eosinophil group.According to disease recurrence,patients in the observation group were divided into recurrence group and non-recurrence group.The expression levels of IL-12 and TGF-β_(1) in different groups were compared.The correlation between IL-12,TGF-β_(1) levels and percentage of eosinophil infiltration was analyzed by Spearman rank correlation analysis.Results The expression levels of IL-12 and TGF-β_(1) in nasal polyp tissues in the observation group were(42.93±10.52) pg/m L and(529.89±147.67) ng/m L,significantly higher than(33.11±9.87) pg/mL,(455.39±134.04) ng/m L in the control group(P<0.05).The expression levels of IL-12 and TGF-β_(1) in mild group were(40.16±9.64) pg/mL and(515.78±65.95) ng/m L,significantly lower than(49.61±9.98) pg/m L,(563.92±57.84) ng/mL in severe group(P<0.05).The expression levels of IL-12 and TGF-β_(1) in eosinophil group were(47.08±10.15) pg/m L and(556.32±74.58) ng/mL,significantly higher than(36.14±10.68) pg/m L,(486.64±81.07) ng/m L in non-eosinophil group(P<0.05).The expression levels of IL-12 and TGF-β_(1) in recurrence group were(48.78±8.35) pg/m L and(571.29±74.84) ng/mL,significantly higher than(40.08±9.06) pg/mL,(509.72±88.65) ng/mL in non-recurrence group(P<0.05).Spearman rank correlation analysis showed that expression levels of IL-12 and TGF-β_(1) were positively correlated with percentage of eosinophil infiltration(P<0.05).Conclusion The expression of IL-12 and TGF-β_(1) in nasal polyp tissues are up-regulated in CRSwNP patients,which are relatively higher in patients with disease progression and severe eosinophil infiltration,and the risk of postoperative disease recurrence is also relatively higher.