石油烃降解菌Mycolicibacterium fluoranthenivorans Y3的筛选鉴定及降解特性研究

Screening,Identification and Degradation Characteristics of Petroleum Hydrocarbon Degrading Bacterium Mycolicibacterium Fluoranthenivorans Y3

从天津港南疆污水处理厂含油污泥中筛选得到一株高效柴油降解菌株Y3,经过形态学观察、生理生化试验、16S rRNA基因序列分析鉴定菌株Y3属于分枝杆菌属(Mycolicibacteriumsp.)。菌株Y3适宜在中温、偏酸性的环境条件下生长,在盐度为3%、Mn^(2+)浓度为50 mg/L的条件下仍可正常生长。该菌株能够耐受3%(V/V)的柴油,最适培养条件下培养5 d后菌株的OD_(600)可达2.0,柴油降解率为50.1%,外加碳酸钠、乙酸钠可提升Y3对柴油的降解能力,气相色谱-质谱(GC-MS)分析表明,Y3可降解柴油(C_(10)-C_(32))中所有的碳氢化合物,对直链烷烃具有较强的降解能力,对个别支链烷烃(2,6,10-三甲基十三烷)降解率达到57.6%,具有良好的石油烃污染修复应用潜力。

A highly efficient diesel degrading strain Y3 was screened from the oily sludge of Nanjiang sewage treatment plant of Tianjin port.Through morphological observation,physiological and biochemical test and 16S rRNA gene sequence analysis,it was identified that strain Y3 belonged to Mycolicibacterium sp.Strain Y3 is suitable to grow under medium temperature and acidic environmental conditions,and can still grow normally under the condition of salinity of 3% and Mn^(2+) concentration of 50 mg/L.The strain can tolerate 3%(V/V) diesel oil.After 5 days under the optimum culture conditions,the OD_(600) of the strain reached 2.0 and the degradation rate of diesel oil was 50.1%.The addition of sodium carbonate and sodium acetate can improve the degradation ability of Y3 to diesel oil.Gas chromatography-mass spectrometry(GC-MS) analysis showed that Y3 could degrade all hydrocarbons in diesel oil(C_(10)-C(32)) and had strong degradation ability to linear alkanes.The degradation rate of individual branched alkanes(2,6,10-trimethyltridecane) is 57.6%,which indicates the application potential to repair petroleum hydrocarbon pollution.