摘要
平菇Pleurotus ostreatus(一种人工栽培的食用真菌)的水提取液作为长期维持梨形四膜虫Tetrahymena pyriformis种源的培养液。在这种培养液中接入对数生长的四膜虫,并在它的上面复盖一层液体石腊油,置室温(温度变化范围为8—30℃)下维持达到一年以上。 用这种方法维持一年后的四膜虫,细胞大小和细胞大核直径都要比在丰富有机培养基中正常生长的对数期细胞要小一些,除外,没有其它明显的形态变化。 用这种方法维持一年后的四膜虫,只需经过一次转接到丰富有机培养基中,其细胞大小、细胞大核直径就能恢复到正常情况,但是转接后发现有较长的停滞期。
A simple medium for Tetrahymena pyriformis, using Pleurotus ostrea-tus, a species of mushroom, as the nutrient source is described. The medium was placed into each tube 5ml, after autoclaved, inoculated with T. pyriformis, and layered over with 2ml of paraffin oil, finally, kept at 8-30℃. T. pyriformis in the medium can be maintained more than one year. T. pyriformis maintained for one year is smaller both in cell size and macronucleic diameter than cells grown in organic medium. Once T. pyriformis maintained with this method was inoculated into usual organic medium, the size of cell and macronucleic diameter was recovered to the same as the normal cells, only their log-phase is longer than normal culture.
作者
沈锡祺
张作人
Shen Xiqi;Zhang Zuoren(Department of Biology,East China Normal University,Shanghai)
关键词
梨形四膜虫
维持方法
维持培养基
平菇
Tetrahymena pyriformis
Maintenanace method
Maintenanace medium
Pleurotus ostreatus
