仔猪白痢毒性大肠杆菌粗制核糖体疫苗的初步研究

IMMUNOPROTECTIVE ACTIVITY OF RIBOSOMAL PREPARATION FROM POISONOUS Escherichia coli,THE PATHOGEN OF PIGLET DYSENTERY

以硫酸铵沉淀的方法制备了仔猪白痢病原体——毒性大肠杆菌的核糖体粗制品,而未使用超速离心设备。本工作表明如此制备的核糖体也同样有很高的免疫保护效力。给小鼠皮下注射50或100微克核糖体15天后,用四倍于全数致死量的活菌作皮下攻击,动物百分之百存活(P<0.001),注射25微克,即已可存活86.7%(P<0.001)。皮下给予核糖体500微克后两个月,进行皮下攻击,动物仍可存活94.7%(P<0.001)。皮下免疫注射后进行腹腔攻击,免疫效果即低得多,此点与文献上肺炎双球菌核糖体的情况相似。

A virulent strain of Escherichia coli had been isolated and found to be the pathogen of piglet dysentery by Mr.Li Xue-liang (Yunnan Institute of Animal Husbandary and Veterinary Medicine).When 6 x 107 living bacteria of ihe virulent strain were injected subcutaneously into each mouse,all the injected mice would be killed.Ribosomal preparations were isolated from disrupted cells by a modification of the ammonium sulfate precipitation method described by C.G.Kurland(1966),omitting the ultracentrifugation step.The ribosomal preparation was proved to be highly immunogcnic for mice and immunization with the preparation elicited significant protective immunity.The effect was obvious when mice immunized subcutancously with ribosomal preparation without adjuvant were challenged subcutancously with 2 -2.4x108 living bacteria two weeks after immunization,ribosomc dose mice challenge dose 30-day survival When immunized mice were challenged subcutaneously with 2.1xl08 living bacteria two months after immunization,immunoprotection was still significant.