一种淡水涡鞭毛虫人工培养成功

THE SUCCESSFUL CULTURE OF A FRESH-WATER DINOFLAGELLATE

于1981年11月初,我们采到一种淡水涡鞭毛虫(甲藻)Glenodinium gymnodinium Penard,随即在实验室进行试探人工培养。所用的培养基为经改良的一个团藻培养基配方。我们采取过渡性的初步培养、纯化培养和扩大培养三步法,利用光强度800—1200勒克斯(Lux),每日照光10—12小时,一般室温在15—22℃(冬季不低于9℃,夏季不高于28℃)下,细胞已完全能正常的生长、繁殖而被培养成功。在对细胞的一些特征进行观察时,发现所培养的光薄甲藻是世界上较为罕见的含有两种不同类型的细胞核的涡鞭毛虫类生物。

In November 1981,we collected a fresh-water dinoflagellate-Glenodinium qymnodinium Penard from a pool in the suburbs of Kunming.We have succeeded in the culture of this organism in the laboratory.The medium used is a modified artificial medium for growing Volvox globartor,devised by Provasoli and Pintner (1959).The process of culture is divided into three steps:(1) preliminary culture;(2) pure culture and (3) expand culture.The work is carried out under 8 -12 hours illumination each day (the intensity of illumination is at 800-1200 Lux) and at temperature 9-26℃ in the common laboratory.So far,the culture of these cells has been carried to more than 300th progeny.After the culture,we also observed the reproduction form,the growth curve and nuclei of this dinoflagellatc,The result showed this organism is a rare dinoflagcllatc both with a mesocaryotic and an cucaryotic nucleus.