摘要
本文研究了豚鼠E花环形成狗淋巴细胞(E-RFC)的类别。狗淋巴细胞于37℃孵育后,可提高E花环形成率。E-RFC与总淋巴细胞的SIg阳性率相近。E-EAC(豚鼠红细胞—抗体和补体包被的绵羊红细胞)混合花环实验表明一部分狗淋巴细胞同时具有E和补体二种受体,总淋巴细胞与补体受体淋巴细胞的E花环阳性率无显著差异。这些结果说明E受体不仅表现于一部分T细胞上,而且见于某些B细胞上。因此,不宜用E花环试验作为检测狗T细胞的方法。
Categories of canine lymphocytes forming rosettes with guinea pig erythro-cytes(E-RFC)were studied.The incubation of canine lymphocytes at 37℃increased E-rosette formation.The percentages of SIg positive cells in E-RFC were similar to those in total lymphocytes.In mixed E-EAC(guinea pig ery-throcytes-sheep erythrocytes coated with antibody and complement)rosette assays,a portion of canine lymphocytes having both E and complement receptors has been detected.No significant difference was found in the mean percentages of E-RFC between total lymphocytes and EAC-rosetting ones.On the basis of these results we assume that the E receptors are present on a part of T cells and also on some B cells.Thus,the E-rosette assay cannot be used for detection of canine T cells.
作者
吕占军
王蕙芬
Lu Zhanjun;Wang Hueifen(Institute of Basic Medicine,Hebei Medical College,Shijiazhuang)
