灵长类淋巴细胞亚群的研究——Ⅰ.外周血和淋巴组织的SRBC、SMRBC和ZYC玫瑰花结形成细胞的测定

STUDIES ON LYMPHOCYTE SUBPOULATIONS IN PRIMATES I. The Determination of SRBC, SMRBC and ZYC Rosette Formation Cell in Peripheral Blood and Lymphoid Tissues

用经过氨乙基异硫脲(AET)或神经氨酸酶(VCN)处理过的绵羊红细胞(SRBC),酵母多糖—补体复合物(ZYC)和红面猴红细胞(SMRBC)作为标记,测定了健康恒河猴、熊猴、豚尾猴、红面猴和树鼩的外周血以及恒河猴淋巴样组织的淋巴细胞亚群,在这五种动物中,E;玫瑰花结形成细胞的百分比依次为78.1±6.4,77.7±4.2,76.0±6.4,82和24.9±7.2。ZYC形成细胞的百分比依次为11.4±4.2,9.0±3.8,12.8±2.2,15和15.7±7.4。恒河猴胸腺,脾脏和淋巴结的E;形成细胞的百分比分别为95.2±2.9,28.8±10.3,44.8±6.2。与人类不同,SMRBC不是恒河猴B淋巴细胞的标记。此外,还与其他作者的结果进行了比较。

The lymphocyte subpapulations in peripheral blod of Macaca mulatta,M.assamensis,M.nemestrina,M,speciosa and Tupaia belangeri and in lymphoid tissues of M.mulatto were determined with markers,such as AET or neurami-nidase treated sheep red blood cell(EABT or EVON),zymosan-yeast-complement complex(ZYC)and stump-tailed m nkey(M.speciosa)red blood cell(SMRBC).The main results of peripheral blood lymphocytes were seen in table 3.The percentages of EAET RFC in thymus,spleen and lymph node of M.mulatto were 95.2±.9,28.8±10.3 and 44.8±.2,respectively.The double marking with ZYC and SMRBC showed that SMRBC rosette formation cells of M.mulatto were not B cells.Finally,the results were compared with those fr jm other authors(see table 5).