恒河猴(Macaca mulatta)肾细胞冻存复苏培养及其生物学特性的研究

STUDY ON FROZEN PRESERVATION AND RESUSCITATION AND BIOLOGICAL CHARACTERISTICS ON PRIMARY DISPERSED TISSUE CELL OF RHESUS MONKEY’S KIDNEY

本文使用深低温(-196℃)冻结保存动物细胞技术,对胰酶分散的恒河猴肾细胞的冻存,复苏培养及其生物学特性进行了研究。结果表明、液氮冻存2—58周的恒河猴肾细胞,其平均存活率为63.3—74.4%。复苏培养细胞于6—7天形成緻密单层,对脊髓灰质炎病毒敏感;国产二甲基亚砜可以用作冻存细胞的保护剂;复苏培养的细胞核型正常;其生物学特性与未冻的原代细胞一致。

Rhesus Monkey kidney trypsin-dispersed tissue cells were preserved at -196.C and resuscitated. The biological characteristics of preserved cells were studied. The results of this study have indicated that cells frozen storage in - 196.C from 2 -53 weeks, had a survival average rates of 63.3-74.4%. The reconstituted cells showed a good morphological appearance and, in general, required 6 -7 days to form a dence mon-olayer, after rewarning at 37.C for reculture. The cell monolayers were very susceptable to polioviruses. Karyotypes and chromosome analyses and sister chromatid exchange assay were unremarkable. Native dimethyl-sulfoxid (DMSO) may be used as a cryo-protective agent.