四种哺乳动物染色体的R带和G带

SIMULTANEOUS DEMOSTRATION OF THE CHROMOSOMAL R- AND G-BANDS IN 4 SPECIES OF MAMMALS

本文描述了一种简化的细胞同步化技术。在培养基中加入5-溴脱氧尿嘧啶核苷(BrdU)使细胞同步化6—8小时,可以得到较多的前期和前中期分裂细胞。经分带染色后,能在一张玻片上同时显出良好的R带和G带。我们采用该技术处理人、赤麂、黑麂、中国仓鼠细胞后得到不同比例的R带和G带,带纹较为清晰,易于辨认,分辨率也比较高,有些染色体的带纹数目要比常规方法所作的R带和G带带纹多。

A simple cell-synchrohization technique by BrdU treatment in vitro was described in this paper.More prophase and prometaphase cells may be obtained by this technique.The chromosomes revealed distinct R-bands and G-bands on the same preparation after special staining.The different proportion of R-bands and G-bands in 4 species of Mammals (human,red muntjak,black muntjak,and Chinese hamster) have been reported.The technique described here may be of interest as an easy method for inducing banding patterns of chromosomes.