茅舍血厉螨核型及染色体的C带、G带的研究

STUDIES ON KARYOTYPE AND C-BANDED,G-BANDED CHROMOSOMES OF Haemolaelaps casalis

本文首次报道了一种革螨——茅舍血厉螨核型及染色体C带、G带的研究。用剖腹取卵法、玻璃纸压片、Giemsa染色,经分析茅舍血厉螨的核型,单倍体n=7,二倍体2n=14。用氢氧化钡—吉姆萨技术显示茅舍血厉螨染色体C带。在第1、2、4、5染色体上出现恒定的C带部分,第3、6、7染色体上出现不恒定的C带部分。根据C带带型,茅舍血厉螨着丝点的位置可分为近中区域(sm),近端区域(St),末端区域(t)和末端点(T)四类。G带分析用胰蛋白酶—吉姆萨技术显示。本文对茅舍血厉螨的核型、革螨染色体研究中螨卵的收集方法和染液的改进、C带带型与着丝粒位置的确定和G带显带问题进行了讨论。

This paper first presents the studies on karyotype and C-banding,G-banding of the chromosome in Haemolaelaps casalis.An analysis of the karyotype of H.casalis was carried out on the basis of the chromosomes of eggs by cellophane squash technique,taking eggs by caesarean section,using Giemsa stain.The results show that the karyotype of H.casalis is haplodiploidy,i.e.,haploid chromosome number is 7(n=7),while that of diploid chromosome is 14(2n=14).C-banding analysis of egg cellophane squashes of H.casalis by barium hydroxide-Giemsa banding technique(CBG)showed that constant C-bands would appear at several positions such as No.1,2,4 and 5 chromosomes,but nonconstant C-bands would do at No.3,6 and 7 chromosomes.According to the result of C-banding pattern,of these chromosomes of H.casalis,No.1,2 and 7 were submetacentric(sm),No.4 was subtelocentric(st),No.6 terminal region centric(t)and No.3 and 5 were terminal point centric(T).The G-banded chromosomes of H.casalis by trypsin-Giemsa banding technique(GTG)evidenced that the position were C-bands have appeared reveal dark bands,with chromosome arms ranging from 2 to 10 dark bands.