摘要
Phenomics explores the complex interactions among genes,epigenetics,symbiotic microorganisms,diet,and environmental exposure based on the physical,chemical,and biological characteristics of individuals and groups.Increasingly efficient and comprehensive phenotyping techniques have been integrated into modern phenomics-related research.Multicolor flow cytometry technology provides more measurement parameters than conventional flow cytometry.Based on detailed descriptions of cell phenotypes,rare cell populations and cell subsets can be distinguished,new cell phenotypes can be discovered,and cell apoptosis characteristics can be detected,which will expand the potential of cell phenomics research.Based on the enhancements in multicolor flow cytometry hardware,software,reagents,and method design,the present review summarizes the recent advances and applications of multicolor flow cytometry in cell phenomics,illuminating the potential of applying phenomics in future studies.
基金
This work was supported by National Key Project of China(2016YFA0502201 and 2017YFA0700404)
Standard Technology Management Project(2013811)
the State Administration for Market Regulation,National Science and Technology Basic Condition Platform project(APT2001)
Natural Science Foundation of Shenzhen(JCYJ20190808150009605).
