摘要
CRISPR/Cas9技术是利用RNA靶向引导Cas9核酸酶对基因组中的目标基因进行编辑的生物技术。近年来,该技术的多种新型基因编辑器更新迅猛,编辑效果愈加精细和高效,在作物定向分子设计育种中展现出巨大的应用前景。该文对CRISPR/Cas9及其相关编辑器的技术原理、编辑效果和应用情况进行综述,并探讨了该技术在应用中面临的问题、应对措施和发展前景,旨在为相关领域的科研工作者提供参考。
CRISPR/Cas9 technology is a gene editing strategy using Cas9 nuclease guided by RNA to target an interest gene in genome. Recently, a large number of new-type gene editors based on the CRISPR/Cas9 have been updated rapidly and become more precisely and efficiently in gene editing, which has a great application prospect in crop molecular-designing breeding. This paper summarized the technical principles, editing effects and applications of the CRISPR/cas9 and its related gene editors, and also discussed the aspects of dilemmas, countermeasures and prospects,intending to provide reference for scientific researchers in the related fields.
作者
何晓玲
刘鹏程
马伯军
陈析丰
Xiaoling He;Pengcheng Liu;Bojun Ma;Xifeng Chen(College of Chemistry and Life Sciences,Zhejiang Normal University,Jinhua 321004,China)
出处
《植物学报》
CAS
CSCD
北大核心
2022年第4期508-531,共24页
Chinese Bulletin of Botany
基金
国家自然科学基金(No.31871605,No.32071987,No.32101697)
浙江省自然科学基金(No.LD19C130001,No.Q22C133851)。
