柞蚕蛹抗菌肽D对大肠杆菌K_(12)D_(31)的作用

THE EFFECT OF CECROPINS D FROM ANTHERAEA PERNYI ON SUSPENSIONS OF E.COLIK12D31

本文报道了不同浓度的柞蚕蛹抗菌肽D,对大肠杆菌KD的杀灭作用动力学。在LEG培养液中,抗菌从D的浓度在5微克/毫升时显效。浓度在10微克/毫升以上时,其杀菌速度大于细菌的增殖速度。固定抗菌肽浓度为10微克/毫升,细菌浓度在3×10^(7)个细菌/毫升,培养在磷酸钾盐缓冲液中,4小时后能全部杀灭。同样浓度细菌在LEG培养液中,4小时后细菌数下降到约为10^(2)个细菌/毫升,但不能全部杀灭。同时还提供了柞蚕蛹抗菌肽D和B对大肠杆菌KD作用不同时间的电镜照片。

The kinetics of the bacteriolytic effect on E.coli K12 D31 were studied at different concentrations of cecropin D from Antheraea pernyi.The effect of 5μg per ml cecropin D on Suspensions of E.Coli K_(12)D_(31)(10^(7)cells/ml)in LEG medium was noticeable.In that medium,When the concentration of cecropin D reached 10μg per ml,the bactericibal velocity was higher than proliferation of bacterial cells As cecropin D was added to the suspension until its concentration reached 10μg per ml and with 10^(7)cells/ml of E.coli K_(12)D_(31),the number of surviving bacteria was counted by spreading on agar plates.It was found that after 4 has of incnhation in 0.1 M potasstum phosphate buffer these were no surivals but in LFG medium the bacterial population was 10^(2)cells/ml.E.coli K_(12)D_(31)which was incubared with cecropin D or B for uifercnt periods was obscrved in the electron microscope.