从提取细胞色素丙后的猪心残渣中制备辅酶Q_(10)的研究

STUDIES ON THE PREPARATION OF COENZYME Q_(10) FROM PIG HEARTS UTILIZED FIRST FOR THE EXTRACTION OF CYTOCHROME C

辅酶Q_(10)(以下简称Q_(10))是一种新的生化药物,有重要的临床用途(中国科学院昆明动物研究所辅酶Q_(10)研究组,1979)。Q_(10)的制备虽可采用化学合成法,但此法难度较大且不经济。自动物、植物、微生物中提取Q_(10)是国外工业生产Q_(10)的主要途径。我国大部分生物制药厂以猪心为原料生产细胞色素丙,因此经提取细胞色素丙后的猪心残渣数量极大。但这些猪心残渣一般被用作肥料或饲料。已知在猪心中Q_(10)含量丰富和Q_(10)在酸性条件下不稳定。(Korner,1974)于是这种经酸性溶液处理的猪心残渣是否还含有未分解的Q_(10),其含量是多少?是综合利用这些猪心残渣的前提。本文证明,这种猪心残渣仍然含有未分解的Q_(10),其含量与新鲜猪心中相同,从而为Q_(10)的工业生产找到了一种来源充足、价廉物美的原料。另外,本文比较并发展了一些Q_(10)的制备方法,为确定Q_(10)的工业生产工艺路线提供了实验依据。最后本文发展了一种Q_(10)的扫描薄层层析分析法,是目前已知各种Q_(10)分析法中较理想的一种。

Pig hearts have been utilized in great quantities for the extraction of cyto-chrome C.In the process of extraction,the pig hearts had to be treated with acidic solution,they thus used to be considered as raff and were discarded.Through a series of analytical studies,we now present evidence that Co Q_(10) is not destroyed in these used pig hearts.They may be utilized as one kind of cheap raw material for the industrial production of Co Q_(10).Various methods for the production of Co Q_(10) were compared.Some non-saponification methods were developed and their advantages over the saponification ones were discussed.A new then layer chromatography method was described.It combines accuracy with simplicity and can be employed both for the qualification and the quantification of Co Q_(10).