不同浓度甘氨酸铁对猪肾细胞铁调基因表达的调节作用

Effects of Different Concentrations of Glycine Iron on the Expression of Iron-Regulated Gene in Pig Kidney Cells

铁是新生动物重要的必需微量元素之一。铁营养对母猪胎儿生长发育和新生仔猪铁代谢具有重要生物学意义。本试验建立猪肾细胞缺铁模型补加甘氨酸铁,通过荧光定量PCR检测促红细胞生成素(Erythropoietin,EPO),低氧诱导因子-1(Hypoxia-inducible factor,HIF-1)和血管内皮生长因子(Vascular endothelial growth factor,VEGF)的基因表达情况,研究甘氨酸铁对猪肾细胞铁代谢基因表达的影响。结果表明,适宜浓度的去铁胺(Deferoxamine,DFO)可有效降低猪肾细胞内铁含量,成功构建猪肾细胞缺铁模型。缺铁细胞添加甘氨酸铁可有效增加细胞内铁含量。添加甘氨酸铁可调控铁代谢基因EPO、HIF-1和VEGF的表达,其信号传导通路需进一步研究。

Iron is one of the essential trace elements in newborn animals.Iron nutrition has important biological sig⁃nificance for the growth and development of sow fetus and the metabolism of newborn pig iron.In this study,Glycine Iron was added to the iron deficiency model of pig kidney cells,and the expression of EPO,HIF-1 and VEGF was detected by fluorescence quantitative PCR to study the effect of Glycine Iron on iron metabolism gene expression in pig kidney cells.The results showed that DFO with appropriate concentration can effectively reduce the content of iron in pig kidney cells,and the model of iron deficiency in pig kidney cells was successfully constructed.Glycine Iron was added to iron deficient cells to effectively increase the iron content in cells.Glycine Iron was added to regu⁃late the expression of iron metabolism genes EPO,HIF-1 and VEGF.Its signal transduction pathways need further study.