云南主栽蓝莓提取液的抑菌活性研究

Study on Antibacterial Activity of Yunnan Blueberry Extracts

本研究以云南主栽不同蓝莓品种为研究对象,以其果实提取液对金黄色葡萄球菌、大肠埃希氏杆菌、铜绿假单胞菌的抑菌效果进行比较研究。结果表明,6个品种蓝莓的提取液中,绿宝石对3种致病菌的抑制效果最差,最小抑菌浓度(MIC)为250 mg/mL,最小杀菌浓度(MBC)为1000 mg/mL。夏普蓝和比洛克西对金黄色葡萄球菌的抑制作用最强,其MIC值和MBC值同为15.625、62.5 mg/mL。密斯提和比洛克西对大肠埃希氏杆菌的抑制作用最强,其MIC值和MBC值同为15.625、62.5 mg/mL,这些蓝莓品种对两种菌的MIC值和MBC值相同,说明它们对两种菌的抑制作用类似。莱格西和灿烂对铜绿假单胞菌的杀菌作用最强,其MBC值为62.5 mg/mL;夏普蓝和比洛克西的MIC值最小,为15.625 mg/mL,灿烂的MBC值最小,为62.5 mg/mL。以此为进一步研究蓝莓花色苷的生理活性奠定理论基础,同时为蓝莓多酚物质应用于食品保鲜提供理论依据。

In this study,different blueberry varieties mainly planted in Yunnan were used as the research object,and the antibacterial effects of their extracts on Staphylococcus aureus,Escherichia coli and Pseudomonas aeruginosa were compared.The results showed that Emerald had the worst inhibitory effect on the three pathogenic bacteria,with the minimum inhibitory concentration MIC of 250 mg/mL and the minimum bactericidal concentration MBC of 1,000 mg/mL.Sharp blue and Biloxi showed the strongest inhibitory effect on Escherichia coli,with the MIC and MBC values of 15.625 mg/mL and 62.5 mg/mL,respectively.The MIC value and MBC value of Misti and Biloxi were 15.625 mg/mL and 62.5 mg/mL,respectively.The MIC value and MBC value of the two blueberry varieties against the two strains were the same,indicating that these blueberry varieties had similar inhibitory effects on the two strains.Legosi and Brilliant had the strongest bactericidal effect on Pseudomonas aeruginosa(MBC),and their MBC value was 62.5 mg/mL.The MIC values of Sharp and Biloxi were the lowest(15.625 mg/mL),while the MBC value of Brilliant was the lowest(62.5 mg/mL).The results provided a theoretical basis for further study on the physiological activity of blueberry anthocyanins,and provided a theoretical basis for the application of blueberry polyphenols in food preservation.