摘要
采用HP-20、聚酰胺和半制备液相等多种色谱手段对卷柏75%乙醇提取物的乙酸乙酯萃取部位的化学成分进行分离纯化,并根据理化性质和波谱数据鉴定其结构,得到1个新的环肽类化合物,命名为卷柏环肽A(1)。采用MTT法测定化合物对B16F10、MDA-MB-231和MDA-MB-468细胞系的抑制活性,化合物1在40μmol·L^(-1)浓度下对B16F10细胞系的抑制率为51.57%±4.34%,在100μmol·L^(-1)浓度下对MDA-MB-231和MDA-MB-468细胞系没有抑制活性。
One new cyclopeptide was isolated from the ethyl acetate fraction of the 75% EtOH extract of Selaginella tamariscina by various column chromatography methods(HP-20, polyamide and semi-preparative HPLC). Its structure was identified as selapeptin A(1) by extensive spectroscopic analysis(HR-ESI-MS, 1 D and 2 D NMR). Compound 1 was evaluated for cytotoxic activities by MTT assay. It showed potent cytotoxic activity against B16 F10 with the inhibition rate of 51.57%±4.34% at 40 μmol·L^(-1)while had no impacts on MDA-MB-231 and MDA-MB-468 at 100 μmol·L^(-1).
作者
阎新佳
温静
宋洋
沙冬梅
沙马里牛
张绍山
刘圆
YAN Xin-jia;WEN Jing;SONG Yang;SHA Dong-mei;SHA Ma-li-niu;ZHANG Shao-shan;LIU Yuan(Institute of Qinghai-Tibetan Plateau,Southwest Minzu University,Chengdu 610041,China;Tibetan Plateau Ethnic Medicinal Resources Protection and Uilization Key Laboratory of National Ethnic Affairs Commssion of the People's Republic of China,Chengdu 610225,China;Sichuan Provincial Qiang-Yi Medicinal Resources Prolection and Uilization Technology Engineering Laboratory,Chengdu 610225,China;Cllege of Pharmacy,Harbin University of Commerce,Harbin 150076,China;Shimadzu(China)Co.,Ld.,Shanghai 200233,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2022年第16期4391-4394,共4页
China Journal of Chinese Materia Medica
基金
国家重点研发计划项目(2018YFC1708005)
四川省科技计划项目(2021YFS0043)
西南民族大学人才类(自科)-引进人才科研启动金项目(RQD2021048)。
关键词
卷柏
环肽
卷柏环肽A
结构鉴定
抗肿瘤活性
Selaginella tamariscina
cyclopeptide
selapeptin A
structural identification
cytotoxicity
