家蚕信使核糖核酸的分离及体外活性测定

SISOLATION OF MESSENGER RNA IN BOMBYX MORI AND IDECTIFICATION OF ITS ACTIVITY IN VITRO

总RNA的制备主要参考Schutz等(1972)的方法,加以修改。除注明外,实验操作控制在4℃以下。以家蚕幼虫的整体 (除去消化道) 为材料,按1:8(W/V)加入pH8.3缓冲液(0.01M Tris,0.075M NaCl,0.005M EDTA,0.5%SDS),置组织捣碎机中迅速匀浆一分钟。