蚕类核酸的研究 1.氯仿去污剂法分离纯化蚕类DNA

Studies of Silkworm (Bombyx mori) Nucleic Acids 1.Isolation and purification of DNA by the method of chloroform and detergent

应用氯仿去污剂的方法,从蚕蛹和幼虫制备DNA,所得DNA制品内没有或者只有微量的RNA和蛋白质。DNA是双链高分子,在蚕类上进行的试验表明这种DNA具有遗传转化作用。

The preparation of DNA was carried out ftom the pupa and larve of silkworm by the method of chloroform and detergent. The DNA preparation obtained was determined to have little or no RNA and protein. DNA is double stranded high molecule. The result of these experiments showed that the DNA preparation was capable of transformation.