用放射免疫分析法对正常狗血浆皮质醇的研究

OBSERVATION ON THE PLASMA CORTISOL(HYDROCORTISONE) OF NORMAL DOGS BY RADIOIMMUNOASSAY METHOD

用放射免疫分析法检测60只正常狗血浆皮质醇浓度。根据狗血浆皮质醇浓度分成了三个类型。上午8时低下午5时高者(30只狗)称为A型,上午8时高下午5时低者(20只狗)称为B型,上午8时与下午5时差不多相等者(10只狗)称为C型。我们发现B型狗血浆皮质醇的分泌有明显的昼夜生物钟现象,呈“U”形。认为呈现三种类型有两个可能性:①可能与狗的种属特性有关;②可能与这些狗的生活习性改变有关。

The plasma cortisol concentrations in 60 normal dogs were determined by radioimmunoassay.According to the plasma cortisol values the dogs might be divided into 3 types,i.e.8:00 AM-low concentration with 5:00 PM-high concentration is called type A (30 dogs),8:00 AM-high concentration with 5:00 PM-low concentratiin is called type B (20 dogs),while 8:00 through 5:00 PM proximal concentration is called type C (10 dogs).We found that the circadian rhythms of the bio-o’clock of plasma cortisol secretion in type B dogs were appeared in"U-form".There were two kinds of causation in these 3 type dogs:(1) The plasma cortisol secretion of dogs were closely depended on the species of dogs.(2) The plasma cortisol secretion of dogs were related with their envirements and their variant "life-afyie".