吉林产蝮蛇(Agkistrodon halys brevicaudus Steingeri)蛇毒的柱层析分离及酶活性和凝血效应的初步测定

IDENTIFICATION OF SOME ENZYMIC AND BLOOD-CLOTTING ACTIVITIES IN THE VENOM OF ACKISTRODON HALYS BREVICAUDUS STEJNEGER AFTER CHROMATOGRAPHIC FRACTIONATION

关于蝮蛇毒的研究,Suzuki和Satake等开始对日本蝮蛇(Agkistrodon halys blomhoffii)蛇毒进行了分离纯化,以后详尽地研究了各组份。近年来,涂光俦等用二乙氨基乙基葡聚糖A-50对浙江产蝮蛇(Agkistrodon halys Pallas)蛇毒进行柱层析分离,得到14个蛋白组份,测定了酶活性并大规模纯化磷酸二酯酶;陈建智等采用类似方法分离蛇岛产蝮蛇(Agkislrodou halya Pallas)蛇毒,得到16个蛋白组份,测定了酶活性和生理活性。

The venom of Agkistrodon halys brevicaudus Stejnegcr from Kirin was fractionated on DEAE-Sephadcx A-50 into 18 different fractions.The experiment conditions are described.Some enzymatic activities(proteinase,arginine esterase,phospholipase A,phosphomonoesterase,L-amino acid oxi-dase)as well as the blood-clotting activities have been determined for each fraction.