CMTM6在胃癌组织中的表达及其与PD-L1和预后的关系

Expression of CMTM6 in gastric cancer tissues and its relationship with PD-L1 and prognosis

目的 分析CMTM6在胃癌组织中的表达,并探讨CMTM6的预后价值及其与PD-L1的关系。方法 采用免疫组织化学法检测82例胃癌组织中CMTM6和PD-L1的表达水平;采用Kaplan-Meier法和Log-rank检验分析CMTM6不同表达的生存差异;采用Pearson相关分析法探讨CMTM6与PD-L1的表达的相关性;采用免疫荧光法检测胃癌组织中CMTM6和PD-L1的荧光表达;采用单变量和多变量Cox比例风险回归模型评估与生存相关的影响因素。结果 免疫组化结果显示,CMTM6和PD-L1在细胞膜和细胞质均有表达。CMTM6表达与年龄、性别、病理类型、肿瘤大小、TNM分期及组织学分级均无关(P>0.05)。PD-L1表达与年龄、性别、病理类型、肿瘤大小、组织学分级无关(P>0.05),而与TNM分期有关(P=0.01)。CMTM6与PD-L1表达具有一定的相关性(r=0.222,P=0.046)。免疫荧光染色结果也显示CMTM6与PD-L1存在共定位。Kaplan-Meier生存分析显示,CMTM6高表达的胃癌患者较CMTM6低表达的胃癌患者生存期缩短(66.8个月vs.74.3个月,P=0.018);单因素和多因素的Cox回归模型分析显示,CMTM6表达可作为判断胃癌患者预后的独立因素(HR=1.989,95%CI:1.286~3.786,P=0.034)。结论 CMTMT6可预测胃癌患者的预后,并与PD-L1表达密切相关。

Objective To evaluate the expression of CMTM6 in gastric cancer tissues,and investigate the prognostic value of CMTM6 and its relationship with PD-L1. Methods The protein expression of CMTM6 and PD-L1 were detected in 82 cases of gastric cancer tissues by immunohistochemical( IHC) assays. Kaplan-Meier survival analysis and Log-rank test were used to compare the survival curves of different CMTM6 expression. The correlation between CMTM6 and PD-L1 expression was determined by Pearson’s correlation. The fluorescence expression of CMTM6 and PD-L1 in gastric cancer tissues were detected by immunofluorescence assays. In addition,the univariate and multivariate Cox proportional hazard regression analyses were used to evaluate the related factors associated with survival. Results Immunohistochemical results showed that CMTM6 and PD-L1 both located mainly at cell membrane and cytoplasm. There was no significant correlation between CMTM6 expression and age,gender,pathological type,tumor size,TNM stage and histological grade( P>0. 05). There was no significant correlation between PD-L1 expression and age,gender,pathological type,tumor size and histological grade( P>0. 05),but correlated with TNM stage( P = 0. 01). CMTM6 expression was positively related to PD-L1( r = 0. 222,P = 0. 046). The results of immunofluorescence double staining also showed that CMTM6 co-localized with PD-L1.Kaplan-Meier survival showed that gastric cancer patients with high expression of CMTM6 had a shorter overall survival than patients with low expression of CMTM6( 66. 8 months vs. 74. 3 months,P = 0. 018). The expression of CMTM6 was an independent risk factor for prognosis in univariate and multivariate Cox proportional hazard regression analyses( HR = 1. 989,95% CI: 1. 286-3. 786,P =0. 034). Conclusion CMTMT6 can predict the prognosis of gastric cancer patients and significantly relates to PD-L1.