透明质酸和聚多巴胺修饰的银纳米粒的制备与表征

Preparation and Characterization of Hyaluronic acid and Polydopamine Modified Silver Nanoparticles

目的:制备透明质酸和聚多巴胺修饰的银纳米粒(PDA-AgNPs@HA),并进行表征评价。方法:利用氧化还原法制备聚多巴胺修饰的银纳米粒(PDA-AgNPs),再进一步修饰透明质酸(HA),构建透明质酸和聚多巴胺修饰的银纳米粒(PDA-AgNPs@HA),并对其形态、粒径、电位、稳定性、紫外光谱分析、光热性能进行表征评价。结果:制备的PDA-AgNPs@HA电镜下为完整的球形,分散性良好;粒径为65.2 nm,电位为-48.73 mV;紫外扫描显示在402 nm和300 nm处有特征吸收峰;体外稳定性和光热性能良好。结论:PDA-AgNPs@HA制备工艺简单,质量可控,具有良好的体外稳定性和光热性能,为抗肿瘤纳米载体的构建提供参考。

Objective To prepare and characterize hyaluronic acid and polydopamine modified silver nanoparticles(PDA-AgNPs@HA).Methods Polydopamine modified silver nanoparticles(PDA-AgNPs)were prepared by oxidation-reduction method,and then were modified with hyaluronic acid further to construct hyaluronic acid and polydopamine modified silver nanoparticles(PDA-AgNPs@HA).The developed PDA-AgNPs@HA was characterized by morphology,particle size,zeta potential,stability,UV-vis spectroscopy and photothermal property.Results PDA-AgNPs@HA was spherical with good dispersion under transmission electron microscopy.The particle size was 65.2 nm with zeta potential of-48.73 mV.It showed characteristic absorption peaks at 402 nm and 300 nm by ultraviolet scanning analysis and exhibited good stability and good photothermal property in vitro.Conclusion PDA-AgNPs@HA was fabricated facilely with controllable quality,and exhibited good stability and photothermal property in vitro,which provided reference for the design of anti-tumor nanocarrier.