指纹图谱结合聚类分析、主成分分析的凉粉草质量评价

Quality Control Study of Mesona Chinensis based on HPLC Fingerprint Combine Principal Component Analysis and Cluster Analysis

目的:通过建立凉粉草药材的高效液相(HPLC)指纹图谱,为科学区分及评价不同来源的凉粉草药材提供参考。方法:采用高效液相色谱仪对凉粉草提取溶液进行梯度洗脱,对比不同来源的凉粉草溶液所得色谱图,选取其中9个特征峰,对其峰面积数据采用相似度分析、主成分分析等进行数据处理。结果:建立凉粉草的指纹图谱,20批次的凉粉草相似度较高,基本大于0.9,统计学分析结果基本一致。结论:来自广东平远上举镇、泗水镇、仁居镇等地的凉粉草质量较好;指纹图谱能较好的区分不同产地的凉粉草,为其质量控制提供科学依据。

Obejective:To provide reference for scientific differentiation and evaluation of Mesona chinensis from different sources by establishing high performance liquid phase(HPLC)fingerprints of Mesona chinensis.Method:Gradient elution was performed on the extract solution of Mesona chinensis by high performance liquid chromatograph.The chromatograms obtained from the solution of Mesona chinensis from different sources were compared,and 9 characteristic peaks were selected,and the peak area data were analyzed by similarity and principal component analysis perform data processing.Results:The fingerprint of Mesona chinensis was established,and the similarity of 20 batches of Mesona chinensis was relatively high,basically greater than 0.9,and the statistical analysis results were basically the same.Conclusion:The quality of the jelly grass from Shangju Town,Sishui Town,Renju Town,Pingyuan,Guangdong Province are better than others;the fingerprint can better distinguish the Mesona chinensis from different origins,and provide a scientific basis for its quality control.