食品级纳米乳液递送姜酮酚的体外生物可及率

In vitro bioaccessibility of paradol in food-grade nanoemulsion-based delivery system

为解决姜酮酚辛辣刺激性强、口服生物利用度低的问题,采用高压均质技术,以菜籽油为载体油、吐温80为乳化剂,制备了姜酮酚纳米乳液,考察了菜籽油质量分数对乳液粒径、Zeta电位、游离脂肪酸释放率和姜酮酚生物可及率的影响。结果表明,随着菜籽油质量分数的提高,纳米乳液粒径显著增大,Zeta电位无明显变化。各乳液小肠消化阶段游离脂肪酸释放率接近100%,油脂基本完全消化,当菜籽油质量分数过低(5%)时,乳液过于黏稠,油滴消化速度减缓。姜酮酚在不同纳米乳液(菜籽油质量分数5%、10%、15%和20%)中的生物可及率均显著高于对照组(未包封姜酮酚)(11.50%±0.20%),分别为61.90%±1.14%、66.80%±1.56%、80.50%±2.50%和86.20%±5.40%,表明纳米乳液递送体系能有效提高姜酮酚的生物可及率,且提升效率与菜籽油质量分数呈正相关。

Nanoemulsions loaded with paradol were prepared by high pressure homogenization technology using rapeseed oil as oil phase and Tween 80 as emulsifier to improve the low oral bioavailability and pungent taste associated with paradol.The effects of rapeseed oil mass fraction on the particle size,Zeta potential,free fatty acid release rate of nanoemulsions and bioaccessibility of paradol were investigated.The result showed that the partide size of nanoemulsions increased significantly with the increase of rapeseed oil mass fraction,while Zeta potential had no obvious change.Meanwhile,free fatty acid release rate of different emulsions in simulated intestinal juice approached up to 100% with almost complete lipid digestion.Emulsion with low rapeseed oil mass fraction(5%) was too viscous resulting in slowdown of lipid digestion rate.The bioaccessibility of paradol encapsulated in nanoemulsions with different mass fraction of rapeseed oil(5%,10%,15% and 20%) were 61.90%±1.14%,66.80%±1.56%,80.50%±2.50% and 86.20%±5.40% respectively,significantly higher than that of the control group without encapsulation(11.50%±0.20%),which indicated that the nanoemulsion delivery system could effectively improve the bioaccessibility of paradol,and the improved efficiency was positively correlated with the mass fraction of rapeseed oil.