超高效液相色谱-串联质谱法测定食品接触用纸和纸制品中乙烯亚胺的迁移量

Determination of Migration of Ethyleneimine in Food Contact Paper and Paper Product by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry

采用超高效液相色谱-串联质谱(UPLC-MS/MS)建立了食品接触用纸和纸制品中乙烯亚胺迁移量的测定方法。以0.1%(体积分数)甲酸水溶液和甲醇作为流动相,使用电喷雾离子源对目标物进行电离,采用多反应监测模式进行测定。结果表明,乙烯亚胺在4%乙酸、10%乙醇、95%乙醇与异辛烷模拟物中的线性范围为0.003~0.05 mg/L,检出限为1μg/L;在橄榄油模拟物中的线性范围为0.003~0.05 mg/kg,检出限为1μg/kg。各食品模拟物中,乙烯亚胺的加标回收率为96.0%~102%,相对标准偏差(RSD)为1.6%~5.9%。采用该方法对20批次实际样品进行检测,有2批次检出乙烯亚胺。该方法简单快速,具有较高的准确性、灵敏度,适用于食品接触用纸和纸制品中乙烯亚胺迁移量的检测。

An analytical method for migration amount of ethyleneimine in food contact paper and paper product was established by ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).Formic acid aqueous solution(0.1%,volume fraction)and methanol were adopted as mobile phases,electrospray ionization(ESI)ion source was used to ionize the target,and multi-reaction monitoring mode was ultilized for detection.The results showed that there was good linear relationships for ethyleneimine in simulants of 4%acetic acid,10%ethanol,95%ethanol and isooctane in the mass concentration range of 0.003-0.05 mg/L,with the detection limits of 1μg/L.Meanwhile,good linear relationship for ethyleneimine in simulant of olive oil existed in the range of 0.003-0.05 mg/kg,with a detection limit of 1μg/kg.The spiked recoveries ranged from96.0%to 102%,with the relative standard deviations(RSDs)of 1.6%-5.9%.20 batches of samples were selected for practical detection,of which 2 batches were found to contain ethyleneimine.The method was sensitive and accurate,and it was suitable for the determination of migration of ethyleneimine in food contact paper and paper products.