绵羊FUT8基因多态性及其与生长性状的关联分析

Polymorphisms of FUT8 Gene and Its Association Analysis with Growth Traits in Sheep

【目的】研究岩藻糖基转移酶8(fucosyltransferase 8,FUT8)基因g.74522417 C>T位点多态性及其对绵羊生长性状的影响,为绵羊分子育种筛选新的遗传标记。【方法】采用Sequenom MassARRAY SNP分型技术、Illumina OvineSNP 600K BeadChip及Illumina OvineSNP 50K BeadChip芯片检测技术对湖羊(n=992)和乌珠穆沁羊群体(n=333)FUT 8基因g.74522417 C>T位点进行分型,并将其多态性与湖羊和乌珠穆沁羊的生长性状进行关联分析。【结果】FUT 8基因g.74522417 C>T位点在乌珠穆沁羊和湖羊群体中均检测出3种基因型:CC、TC和TT。群体遗传分析表明,g.74522417 C>T位点在乌珠穆沁羊和湖羊群体中均处于中度多态(0.25<PIC<0.50)。卡方适应性检验结果表明,g.74522417 C>T位点在乌珠穆沁羊和湖羊群体中均处于哈代-温伯格平衡状态(P>0.05)。关联分析结果表明,FUT 8基因g.74522417 C>T位点与乌珠穆沁羊4月龄体长和6月龄体高均呈显著相关(P<0.05);与湖羊3月龄管围、5月龄腰角宽和管围、6月龄眼肌面积和管围均呈显著或极显著相关(P<0.05;P<0.01)。【结论】FUT 8基因g.74522417 C>T位点可作为绵羊生长发育潜在分子标记,为FUT 8基因在绵羊分子育种中的应用提供参考。

【Objective】This study was aimed to explore the polymorphism of fucosyltransferase 8(FUT8)gene g.74522417 C>T and its effect on the growth traits in sheep,and screen the new genetic markers that could be used for molecular breading in sheep.【Method】The g.74522417 C>T of FUT 8 gene in Hu sheep(n=992)and Ujumqin sheep(n=333)were typed by Sequenom MassARRAY■ SNP typing technology,Illumina OvineSNP 600K BeadChip and Illumina OvineSNP50K BeadChip chip detection technology,and analyzed the association between g.74522417 C>T and growth traits in Hu and Ujumqin sheep.【Result】There were three genotypes(CC,TC and TT)of FUT 8 gene g.74522417 C>T in Hu and Ujumqin sheep,respectively.Population genetic analysis showed that g.74522417 C>T was in moderate polymorphism in Hu and Ujumqin sheep(0.25<PIC<0.50).The result ofχ2 test showed that g.74522417 C>T was in Hard-Weinberg equilibrium in two sheep population(P>0.05).Association analysis revealed that FUT 8 gene g.74522417 C>T was significantly associated with body length at 4 months of age and body height at 6 months of age in Ujumqin sheep(P<0.05),was significantly or extremely significantly associated with shin circumference at 3 months of age,waist angle width and shin circumference at 5 months of age,and loin muscle area and shin circumference at 6 months of age in Hu sheep(P<0.05 or P<0.01).【Conclusion】FUT 8 gene g.74522417 C>T could be used as a potential molecular marker for growth and development in sheep,providing a reference for the application of FUT 8 gene in molecular breeding of sheep.