血清HIF-1α、CK-BB水平与新生儿缺氧缺血性脑病严重程度的相关性

Correlation between serum HIF-1α,CK-BB levels and the severity of neonatal hypoxic ischemic encephalopathy

目的 探讨血清低氧诱导因子-1α(HIF-1α)、肌酸激酶BB同工酶(CK-BB)与新生儿缺氧缺血性脑病(HIE)严重程度的相关性。方法 选取HIE患儿230例(HIE组),根据病情严重程度分为轻度(102例)、中度(71例)、重度(57例)。以健康新生儿200名作为正常对照组。检测所有对象血清HIF-1α、CK-BB水平,并采用20项新生儿行为神经测定评分法(NBNA)评估脑损伤的严重程度。采用Pearson相关分析评估血清HIF-1α、CK-BB水平与NBNA评分的相关性。结果 HIE组血清HIF-1α、CK-BB水平均高于正常对照组(P<0.001),NBNA评分低于对照组(P<0.001)。血清HIF-1α、CK-BB水平与NBNA评分均呈负相关(r值分别为-0.701、-0.683,P<0.05)。随着病情严重程度的增加,HIE患儿血清HIF-1α、CK-BB水平依次升高(P<0.001),NBNA评分依次降低(P<0.001)。结论 血清HIF-1α、CK-BB或可作为HIE患儿病情严重程度的评估指标。

Objective To investigate the correlation between serum hypoxia inducible factor-1 alpha(HIF-1α)and creatine kinase isoenzyme-BB(CK-BB)and the severity of neonatal hypoxic ischemic encephalopathy(HIE).Methods A total of 230 neonates with HIE were enrolled and classified into 102 cases of mild,71 cases of moderate and 57 cases of severe according to the disease severity. Totally,200 healthy neonates were enrolled as healthy control group. Serum levels of HIF-1α and CK-BB were determined,and the 20 items of neonatal behavioral neurological assessment(NBNA) score were used to evaluate the severity of brain injury. Pearson correlation analysis was used to evaluate the correlation between serum HIF-1α,CK-BB levels and NBNA score. Results The levels of serum HIF-1α and CK-BB in neonatal HIE group were higher than those in healthy control group(P<0.001),and the NBNA score was lower than that in healthy control group(P<0.001). Serum HIF-1α and CK-BB levels were negatively correlated with NBNA scores(r=-0.701 and-0.683,P<0.05). In neonatal HIE group,with the increase of disease severity,the levels of serum HIF-1α and CK-BB were increased in turn(P<0.001),and the NBNA scores were decreased in turn(P<0.001). Conclusions Serum HIF-1α and CK-BB may be used as the indicators for evaluating the disease severity in HIE neonates.