江西省布鲁菌分离株的鉴定及分子特征分析

Identification and molecular characterization analysisof Brucella strains isolated from human in Jiangxi province

目的对江西省近几年分离的布鲁菌进行鉴定和分型,同时检测rpoB基因的携带情况并分析其遗传多态性。方法利用BCSP31-PCR对分离的菌株进行布鲁菌鉴定,利用AMOS-PCR对布鲁菌进行种型分析,利用普通PCR对rpoB基因进行检测,将rpoB基因的PCR扩增产物进行测序并用MEGA6.0软件进行多态性分析。结果经鉴定32株布鲁菌全部为羊种布鲁菌,通过对rpoB基因的PCR扩增产物序列分析显示羊种布鲁菌rpoB基因同源性较高。结论江西省近年人感染的布鲁菌均为羊种布鲁菌,且近几年人感染的羊种布鲁菌rpoB基因高度同源。

Objective To identify and type Brucella isolated from Jiangxi Province in recent years,and at the same time,detect the rpoB gene and analyze the genetic polymorphism.Methods The isolated strains were identified by BCSP31-PCR,the type of Brucella was analyzed by AMOS-PCR,and the rpoB gene was detected by ordinary PCR,the PCR amplification products of rpoB gene were sequenced and the MEGA6.0 software was used for its polymorphism analysis.Results All 32 strains of Brucella were identified as Brucella melitensis.The sequence analysis of the PCR amplification products of the rpoB gene showed that the rpoB gene of Brucella melitensis had high homology.Conclusion The Brucella species infected by humans in Jiangxi Province in recent years were all Brucella melitensis,and the rpoB gene of the human-infected Brucella melitensis in recent years was highly homologous.