光温条件对苍术光合荧光参数及挥发油含量的影响

Effect of Light and Temperature on Photosynthetic Fluorescence Parameters and Volatile Oil Content of Atractylodes lancea

目的:研究不同温度和光强处理对苍术光合荧光参数以及挥发油含量的影响,探索温度和光照共同作用对苍术生长和挥发油积累产生的影响,为合理选择苍术栽培环境提供借鉴。方法:设置32、22℃2个温度处理和全光照、遮阴2个光照处理,测定苍术净光合速率、水分利用效率、羧化速率等光合指标,以及光响应曲线、CO_(2)响应曲线、荧光参数,并测定苍术4种挥发油含量。结果:高温强光下苍术净光合速率、水分利用效率显著高于高温弱光和适温强光,适温弱光下苍术光能利用力最高,而强光下苍术对弱光的利用率显著降低;适温较高温下苍术光合速率易受光强变化和CO_(2)浓度变化的影响,弱光下最大光合速率、表观量子效率等显著高于强光;高温下光反应效率高于适温;高温弱光处理的挥发油总量最高,达到4.582%,高温弱光较高温强光显著增加茅苍术醇和β-桉叶醇含量,分别增加近91.7%和35.7%,而适温强光较高温强光显著增加茅苍术醇含量,增加近87.5%,适温弱光处理的β-桉叶醇含量较高温弱光处理显著降低。结论:苍术生长受到温度和光照的交叉互作,挥发油积累所需光温条件与其生长发育适宜光温条件不一致,苍术通过调控挥发油合成积累来响应光温条件变化。

Objective:To study the effect of temperature and light intensity on photosynthetic fluorescence parameters,volatile oil content,and growth of Atractylodes lancea and provide reference for the rational selection of cultivation environment for A.lancea.Method:We determined the photosynthetic indexes(such as net photosynthetic rate,water use efficiency,and carboxylation rate),light response curve,CO_(2) response curve,fluorescence parameters,and the content of four volatile oils in A.lancea under two temperature treatments(32°C and 22°C)and two light treatments(full light and shade).Result:The net photosynthetic rate and water use efficiency of A.lancea under high temperature+strong light were significantly higher than those under high temperature+weak light and low temperature+strong light.The ability of A.lancea to use weak light at low temperature was the strongest,while the utilization rate of weak light under strong light significantly reduced.The photosynthetic rate of A.lancea at low temperature was more susceptible to light intensity and CO_(2) concentration than that at high temperature.The maximum photosynthetic rate and apparent quantum efficiency under weak light were significantly higher than those under strong light.The photoreaction efficiency at high temperature was higher than that at low temperature.The total amount of volatile oil in A.lancea treated with high temperature+weak light was the highest,reaching 4.582%.Compared with high temperature+strong light,high temperature+weak light significantly increased the content of hinesol andβ-eudesmol in A.lancea by91.7%and 35.7%,respectively,and low temperature+strong light significantly increased the content of hinesol by 87.5%.The content ofβ-eudesmol in low temperature+weak light treatment was significantly lower than that in high temperature+weak light treatment.Conclusion:TThe growth of A.lancea was affected by the interaction between temperature and light.The light and temperature conditions required for the accumulation of volatile oil were not consistent with those suitable for the growth and development of A.lancea.A.lancea responded to the changes of light and temperature conditions by regulating the synthesis and accumulation of volatile oil.