中脑星形胶质细胞源性神经营养因子在人、大鼠及小鼠脑中的表达分布

Distribution of mesencephalic astrocyte derived neurotrophic factor in human,mouse and rat brain

目的探讨中脑星形胶质细胞源性神经营养因子(MANF)在人、大鼠和小鼠脑中的表达分布。方法使用免疫组织化学染色方法,在5例人脑标本、6只小鼠及6只大鼠中检测MANF在人、大鼠和小鼠脑中的表达分布情况并比较物种间差异性。结果MANF在人、大鼠及小鼠脑中的表达水平具有脑区差异性。在人脑中,MANF在大脑皮层的表达水平最高,在皮层下区域,脑干和小脑的表达水平稍弱。MANF在大鼠和小鼠脑内的表达分布与人脑略有不同,但总体一致。结论MANF在人、大鼠和小鼠脑中的表达情况大致相同,但在物种内部,MANF在不同脑区中表达水平存在差异。

Objective To observe the distribution of mesencephalic astrocyte derived neurotrophic factor(MANF)within the brain in human,mice and rats and the comparison across the species.Methods An immunohistochemical method was applied to investigate the expression of MANF and to compare the differences among species in the brain of human specimens(n=5),mice(n=6),and rats(n=6).Results The expression of MANF varied in different brain regions in human,mice and rats.In human,in the cortex,the expression of MANF was the highest among all brain regions.In the subcortical areas or the nucleus,brainstem and cerebellum expression level of MANF were relatively lower.The distribution of MANF in mouse and rat brain were slightly different from that in human brain,but generally consistent.Conclusion The distribution pattern of MANF is similar across the species,however,within a species,MANF expression levels varies in different brain regions.