月季中R2R3-MYB基因RhMYB113c调控花青素苷合成

Analysis the Function of R2R3 MYB Transcription Factor RhMYB113c on Regulating Anthocyanin Synthesis in Rosa hybrida

以月季红色花品种‘卡罗拉’(Rosahybrida‘Carola’)为材料,从其花瓣cDNA中克隆到1个R2R3-MYB基因,命名为RhMYB113c。其开放阅读框为699 bp,编码233个氨基酸。蛋白多重序列比对分析发现RhMYB113c含有R2和R3结构域。系统进化树分析表明,RhMYB113c与花青素苷合成激活因子AtMYB114、AtMYB113、At MYB75、AtMYB90亲缘关系最近。组织特异性表达检测发现,RhMYB113c主要在‘卡罗拉’花瓣和花柱中表达,在白色花品种‘白缎’所有组织中均不表达。不同花发育时期的基因表达显示,RhMYB113c在‘卡罗拉’未着色花瓣中不表达,在盛花期花瓣中高表达,而在白色品种的每个时期均不表达。酵母双杂交和烟草瞬时表达试验证实RhMYB113c调控花青素苷的合成,从而影响月季红色花瓣着色。

An R2R3 MYB gene,named RhMYB113c,was cloned from cDNA of the petal in Rosa hybrida ’Carola’. The open reading frame of RhMYB113c was 699 base pair,encoding 233 amino acids.The multiple sequence alignment analysis showed that RhMYB113c contained R2 and R3 domains.Phylogenetic tree results indicated that RhMYB113c was closely related to anthocyanin synthesis activator AtMYB114,AtMYB113,AtMYB75,and AtMYB90. Tissue specific expression showed that RhMYB113c was mainly expressed in petals and styles in ’Carola’,but not in all tissues of white flower variety ’White Satin’. Gene expression at different flower development stages indicated that RhMYB113c was not expressed in ’Carola’uncolored petals,highly expressed in full flowering stage,but not in all stage of white flower varieties ’White Satin’. Yeast two hybrid and tobacco transient expression experiments suggested that RhMYB113c may regulate anthocyanin synthesis and determine the color of rose petals.