肝癌组织内TLR4/MyD88/NF-κB表达与根治术预后的相关性分析

Correlation analysis of TLR4/MyD88/NF-κB expression in liver cancer tissue and prognosis after radical resection

目的分析肝细胞癌(HCC)组织内Toll样受体4(TLR4)/髓样分化因子88(MyD88)/核因子-κB(NF-κB)表达与根治术预后的相关性。方法采用回顾性研究,选取2019年1月至2021年1月解放军联勤保障部队第910医院收治且经病理确诊为HCC的89例患者为研究对象,采用免疫组织化学法检测肝癌组织及癌旁组织标本中TLR4、MyD88、NF-κB水平,分析TLR4、MyD88、NF-κB与患者临床病理特征和生存率的关系。结果肝癌组织中TLR4、MyD88、NF-κB的阳性表达率为87.64%、85.39%、80.90%,均高于癌旁组织(11.24%、11.24%、26.97%),差异均有统计学意义(P<0.05)。TLR4、MyD88、NF-κB阳性患者的中低分化、TNM分期Ⅲ~Ⅳ期、包膜不完整、有门静脉癌栓、Child-puge分级为B级、甲胎蛋白表达阳性的占比均高于TLR4、MyD88、NF-κB阴性患者,差异均有统计学意义(P<0.05)。89例患者随访至2022年1月31日,各组均5例失访,随访率为94.38%,TLR4、MyD88、NF-κB阳性组患者的3年生存率为12.33%、12.50%、10.29%,均低于阴性组患者(54.55%、50.00%、50.00%),差异均有统计学意义(P<0.05)。结论HCC患者肝癌根治术后预后与TLR4/MyD88/NF-κB通路作用相关,TLR4、MyD88、NF-κB阳性表达者的预后较差。

Objective To analyze the correlation between the expression of Toll-like receptor 4(TLR4)/myeloid differentiation factor88(MyD88)/nuclear factor-κ(NF-κB)in liver cancer tissues and the prognosis of radical resection.Methods A total of 89 patients who were admitted to the 910th Hospital of the Joint Logistics Support Force of People’s Liberation Army from January 2019 to January 2021 pathologically and diagnosed with primary liver cancer(HCC)by pathology were selected as the research subjects,and immunohistochemistry was used to detect TLR4,levels of MyD88 and NF-κB,and the relationship between TLR4,MyD88,NF-κB and clinicopathological characteristics and survival rate of patients was analyzed.Results The positive expression rates of TLR4,MyD88,and NF-κB in liver cancer tissue were 87.64%,85.39%,80.90%,which were higher than those in adjacent tissue(11.24%,11.24%,26.97%),the differences were statistically significant(P<0.05).TLR4,MyD88,and NF-κB positive patients were moderately or poorly differentiated,TNM stageⅢ-Ⅳ,incomplete capsule,and had portal vein cancer.The proportions of suppositories,Child-puge grade B,and positive alpha-fetoprotein expression were higher than those of TLR4,MyD88,and NF-κB negative patients,the differences were statistically significant(P<0.05).Eighty-nine patients were followed up until January 31,2022.5 patients in each group were lost to follow-up,with a follow-up rate of 94.38%.The 3-year survival rates of patients in the TLR4,MyD88,and NF-κB positive group were 12.33%,12.50%,10.29%,which were lower than that in the negative group(54.55%,50.00%,50.00%),the differences were statistically significant(P<0.05).Conclusion The prognosis of HCC patients after radical resection of liver cancer is related to the role of TLR4/MyD88/NF-κB pathway,and the prognosis of patients with positive expression of TLR4,MyD88 and NF-κB is poor.