NKG2D基因多态性与儿童毛细支气管炎易感性及外周炎症细胞因子的关系

Relationship between NKG2D gene polymorphism and susceptibility to bronchiolitis and peripheral inflammatory cytokines in children

目的探讨NKG2D基因多态性与儿童毛细支气管炎易感性及外周炎症细胞因子的关系。方法选取2019年12月至2021年12月该院收治的120例毛细支气管炎患儿作为病例组,另选取同期该院门诊行健康体检的98例健康儿童作为对照组。检测两组血常规及一般生化指标,采用实时荧光定量聚合酶链反应检测NKG2D基因rs48965单核苷酸多态性,采用酶联免疫吸附试验检测血清炎症因子干扰素(IFN)-γ、白细胞介素(IL)-4、免疫球蛋白(Ig)E水平。采用多因素Logistic回归分析NKG2D基因rs48965多态性位点的基因型与毛细支气管炎的易感性,以OR及95%CI表示相对风险度。结果病例组NKG2D rs48965 AA基因型比例(66.7%)高于对照组(35.7%),NKG2D rs48965 TT基因型比例(18.3%)低于对照组(55.1%),NKG2D rs48965 AA基因型能增加儿童毛细支气管炎的易感性(OR=1.93,95%CI:1.25~2.93,P<0.05)。病例组NKG2D rs48965 A等位基因频率比例(74.2%)高于对照组(40.3%),NKG2D rs48965 T等位基因频率比例(25.8%)低于对照组(59.7%),NKG2D rs48965 A等位基因频率能增加儿童毛细支气管炎的易感性(OR=2.29,95%CI:1.62~3.20,P<0.05)。病例组白细胞计数、γ-谷氨酰转移酶、IFN-γ、IL-4、IgE水平均高于对照组,差异均有统计学意义(P<0.05)。NKG2D rs48965 AA基因型患儿IFN-γ、IL-4、IgE水平均高于AT、TT基因型患儿,差异均有统计学意义(P<0.05);NKG2D rs48965 AT基因型患儿IFN-γ、IL-4、IgE水平均高于TT基因型患儿,差异均有统计学意义(P<0.05)。多因素Logistic回归分析结果显示,NKG2D rs48965 AA基因型、NKG2D rs48965 A等位基因频率比例增高为儿童毛细支气管炎发生的独立危险因素(P<0.05)。结论NKG2D rs48965 AA基因型、NKG2D rs48965 A等位基因能增加儿童毛细支气管炎的易感性,并且与外周炎症细胞因子具有一定的相关性。

Objective To investigate the relationship between NKG2D gene polymorphism and susceptibility to bronchiolitis and peripheral inflammatory cytokines in children.Methods A total of 120 children with bronchiolitis who were treated in the hospital from December 2019 to December 2021 were selected as the case group,and 98 healthy children who underwent physical examinations in the outpatient clinic of the hospital were selected as the control group.The blood routine test and general biochemical indexes of the two groups were detected.The single nucleotide polymorphism of NKG2D gene rs48965 was determined by real time fluorescence quantitative polymerase chain reaction,and the levels of serum inflammatory factors interferon(IFN)-γ,interleukin(IL)-4 and immunoglobulin(Ig)E were detected by enzyme linked immunosorbent assay.Multivariate Logistic regression analysis was used to investigate the genotype of NKG2D gene rs48965 polymorphism and the susceptibility to bronchiolitis.The relative risk was expressed as OR and 95%CI.Results The proportion of NKG2D rs48965 AA genotype in the case group was higher than that in the control group(66.7%vs.35.7%),and the proportion of NKG2D rs48965 TT genotype was lower than that in the control group(18.3%vs.55.1%),and NKG2D rs48965 AA genotype increased the susceptibility to bronchiolitis in children(OR=1.93,95%CI:1.25-2.93,P<0.05).The NKG2D rs48965 A allele frequency in the case group was higher than that in the control group(74.2%vs.40.3%),the NKG2D rs48965 T allele frequency in the case group was lower than that in the control group(25.8%vs.59.7%),and the NKG2D rs48965 A allele frequency increased the susceptibility to bronchiolitis in children(OR=2.29,95%CI:1.62-3.20,P<0.05).The white blood cell count,glutamyltranspeptidase,IFN-γ,IL-4 and IgE levels in the case group were higher than those in the control group,and the differences were statistically significant(P<0.05).Children with NKG2D rs48965 AA genotype had higher levels of IFN-γ,IL-4 and IgE than those with AT and TT genotypes,and the differences were statistically significant(P<0.05).Children with NKG2D rs48965 AT genotype had higher levels of IFN-γ,IL-4 and IgE in children with TT genotype,and the differences were statistically significant(P<0.05).Multivariate Logistic regression analysis showed that NKG2D rs48965 AA genotype and the increase of NKG2D rs48965 A allele frequency were risk factors for bronchiolitis in children(P<0.05).Conclusion NKG2D rs48965 AA genotype and NKG2D rs48965 A allele could increase the susceptibility to bronchiolitis,and have a certain correlation with peripheral inflammatory cytokines.