烟曲霉cyp51A基因序列BLAST数据库的建立

Construction of BLAST database of Aspergillus fumigatus cyp51A gene sequence

目的构建我国烟曲霉cyp51A基因序列BLAST数据库。方法对本课题组收集的143株耐药与非耐药临床烟曲霉进行cyp51A基因测序,使用BLAST工具包汇总测序结果,构建本地数据库。结果此BLAST数据库包含143株耐药与非耐药烟曲霉cyp51A基因序列,以1株耐药与1株非耐药待测烟曲霉标本的cyp51A序列对此数据库进行检索和比对可以确定烟曲霉cyp51A基因型。结论本数据库有望用于cyp51A基因的检索与比对,鉴定烟曲霉cyp51A耐药基因型。

Objective To construct BLAST database of Aspergillus fumigatus cyp51A gene sequence.Methods The cyp51A genes of the 143 drug-resistant and non-drug-resistant clinical Aspergillus fumigatus strains collected by our group were sequenced,and the sequencing results were put together to construct a local BLAST database.Results This BLAST database contains cyp51A gene sequences of 143 drug-resistant and non-drug-resistant Aspergillus fumigatus strains.cyp51A sequences of Aspergillus fumigatus specimens can be confirmed by searching this database.Conclusion This BLAST database can be used for search and comparison of cyp51A gene to identify cyp51A drug-resistant genotypes of Aspergillus fumigatus.