高效液相色谱法测定糕点中脱氢乙酸的不确定度评定

Evaluation of Uncertainty in the Determination of Dehydroacetic Acid in Cakes by High Performance Liquid Chromatography

目的:通过高效液相色谱法测定市售糕点中脱氢乙酸,对不确定度的来源和影响因素进行全面分析。方法:根据脱氢乙酸测定过程,构建数学模型展开分析,确定测定过程不确定度的主要来源。结果:合成不确定度为2.436 75 mg·kg^(-1),扩展不确定度为4.873 5 mg·kg^(-1)。结论:仪器稳定性、前处理过程及称量过程是不确定度的主要来源,测定过程中应重点关注。

Objective: Determination of dehydroacetic acid in commercial cakes by high performance liquid chromatography, and a comprehensive analysis of the sources and influencing factors of uncertainty. Method: According to the determination process of dehydroacetic acid, a mathematical model was constructed to carry out analysis to determine the main source of uncertainty in the determination process. Result: The combined uncertainty was 2.436 75 mg·kg^(-1), and the expanded uncertainty was 4.873 5 mg·kg^(-1). Conclusion: The instrument stability, pretreatment process and weighing process are the main sources of uncertainty and should be focused on in the determination process.