碳硼烷基苯并咪唑鎓环蕃的合成及核苷酸识别性质研究

Synthesis and Nucleotide Recognition Properties of Carborane-Based Benzoimidazolium Cyclophane

以1,2-双(4-苄溴苯基)-邻碳硼烷为原料合成了两种新型的碳硼烷基苯并咪唑鎓类荧光探针4a和4b,通过IR,1H NMR,13C NMR,11B NMR和ESI-MS对其结构进行表征,并通过荧光、紫外光谱研究了探针4a和4b对核苷酸的识别性能.荧光滴定结果表明:探针4a和4b可以对单磷酸尿苷(UMP)实现on-off型响应,并具有良好的选择性和灵敏度,其中探针4b对于UMP的检测限可以达到3.41×10^(-8)mol/L.通过Job’s曲线和1HNMR滴定对探针4b的识别机理进行了初步的研究.

Two novel carborane-based benzimidazolium fluorescent probes 4 a and 4 b were synthesized using 1,2-bis(4-benzylbromophenyl)-o-carborane as starting materials.The structures were characterized by IR,1 H NMR,13 C NMR,11 B NMR and ESI-MS,and the recognition properties of probes 4 a and 4 b for nucleotides were studied by fluorescence and ultraviolet spectroscopy.The results of fluorescence titration showed that probes 4 a and 4 b could achieve on-off response to uridine monophosphate(UMP),and had good selectivity and sensitivity.The detection limit of probe 4 b for UMP could reach 3.41×10^(-8)mol/L.The recognition mechanism of the probe 4 b was studied by Job’s curve and 1 H NMR titration.