重组胶原蛋白的产业发展历程和生物医学应用前景展望

Industrial development and biomedical application prospect of recombinant collagen

重组胶原蛋白作为天然动物组织胶原的替代物具有广泛应用于生物材料、生物医学等领域的潜力。种类繁多的重组胶原蛋白类型及其衍生体在多种表达系统中可实现一定规模的产业化生产,为探索和拓展重组胶原蛋白的临床应用奠定了基础。文中简述了重组胶原蛋白的不同表达体系,如大肠杆菌、酵母、植物、昆虫、哺乳动物和人类细胞表达体系,重组胶原蛋白的优势及潜在的应用和局限。着重介绍了目前重组胶原蛋白生产,包括不同表达体系的构建策略和重组胶原蛋白羟基化修饰等方面的研究进展,总结了重组胶原蛋白在生物医药领域的应用及应用基础研究和应用前景展望。

Recombinant collagen,as an alternative to natural collagen,has the potential to be widely used in biomaterials,biomedicine,etc.Diverse recombinant collagens and their variants can be industrially produced in a variety of expression systems,which lays a foundation for exploring and expanding the clinical application of recombinant collagens.We reviewed different expression systems for recombinant collagens,such as prokaryotic expression systems,yeast expression systems,as well as plant,insect,mammal,and human cell expression systems,and introduced the advantages,potential applications,and limitations of recombinant collagen.In particularly,we focused on the current progress in the recombinant collagen production,including recombinant expression system construction and hydroxylation strategies of recombinant collagen,and summarized the current biomedical applications of recombinant collagen.