利用醋糟生物纯化木质素新策略的效能评估

Effectiveness assessment of a new strategy for the biopurification of lignin from vinegar residues

木质素是醋糟的重要组成部分,是自然界中可直接提供芳香环结构物质的可再生资源,具有广泛的用途。然而,醋糟中木质纤维素结构稳定且难以破坏,限制了木质素的生物分离纯化效率,进而影响了醋糟的资源化利用。针对此问题,设计了一种新的木质素生物分离纯化策略,将混合真菌发酵与厌氧消化处理相结合,借助不同功能微生物的协同作用,在有效破坏木质纤维素结构的同时充分降解多糖。结果表明,经过处理后,醋糟中纤维素和半纤维素的降解率达到了87.65%和96.34%;木质素的纯度达到了62.32%,回收率为76.01%。醋糟中木质纤维素结构被破坏,从而导致体系中水解酶活性的提高。包含GH43、CE1、GH13等碳水化合物活性酶基因的微生物的大量富集是该方法实现木质素高效纯化的根本原因。本研究结果可为醋糟的资源化利用提供参考。

As an important component of vinegar residues, lignin is a renewable resource for providing aromatic substances in nature, and it can be widely used. However, the lignocellulosic structure of vinegar residues is very stable and the difficulty in destroying this structure leads to the low efficiency for lignin separation and purification with biological methods, and further affects the utilization of vinegar residues. For solving this problem, a novel strategy for lignin separation and purification was proposed. Based on the combination of mixed fungal fermentation and anaerobic digestion treatment process, the efficient purification of lignin can be attained through the synergistic effect of effective destruction of lignocellulosic structure and degradation of polysaccharides by microorganisms with different functions. After treatment, the degradation rate of cellulose and hemicellulose in vinegar residues reached 87.65% and 96.34%, the purity of lignin was 62.32%and the recovery rate was 76.01%. The increase of hydrolase activity and the enrichment of microorganisms containing carbohydrate-active enzyme(GH43, CE1 and GH13) genes in the treatment system after destruction of lignocellulosic structure of vinegar residues were the fundamental reason for the efficient purification of lignin by this strategy. The results showed the great potential of this strategy for the efficient separation and purification of lignin from vinegar residues, which can provide a reference for the resource utilization of vinegar residues.