无机杂化脂肪酶的制备及其催化性能研究

Preparation and Catalytic Properties of Inorganic Hybrid Lipase

采用脂肪酶为有机组分,Ca^(2+)、Zn^(2+)、Mn^(2+)和Cu^(2+)等4种金属磷酸盐为无机组分,制备脂肪酶-无机杂化纳米花,系统研究4种杂化脂肪酶的结构和催化活性.结果表明,制备的杂化脂肪酶具有花状的层级片状结构,并探究了无机杂化脂肪酶的最优反应时间为10 min,反应温度为40℃,pH值为8.4种金属离子制备的杂化脂肪酶包封率达到90%以上.相比于游离脂肪酶,杂化脂肪酶的催化水解活性、催化反应速率、耐温性、耐变性均有所提高,其中Ca^(2+)金属磷酸盐合成的杂化脂肪酶催化效率是游离脂肪酶的1.87倍,合成的杂化脂肪酶表现出良好的重复使用性.

Lipase-inorganic hybrid nanoflowers were prepared by using lipase as the organic component and four metal phosphates including Ca^(2+),Zn^(2+),Mn^(2+)and Cu^(2+)as the inorganic component,and the structure and catalytic activity of the four hybrid lipases were systematically studied.The results show that the prepared hybrid lipase has a flower-like hierarchical lamellar structure,and the optimal catalytic conditions of inorganic hybrid lipase were as follows:recation time 10 min,reaction temperature 40℃,and pH=8.The encapsulation efficiency of the hybrid lipase reaches more than 90%.Compared with free lipase,the catalytic hydrolysis activity,the catalytic reaction rate,the temperature resistance,and the denaturant resistance of hybrid lipase were improved.The catalytic efficiency of hybrid lipase for Ca^(2+)metal phosphate synthesis is 1.87 times that of free lipase.The synthetic hybrid lipase showed good reusability.