维生素D对妊娠期高血压大鼠肝脏的保护作用及机制

A study of the protective effect of vitamin D on liver in hypertensive rats during pregnancy

目的观察维生素D对妊娠期高血压大鼠肝脏的影响,探讨维生素D对妊娠期高血压大鼠肝损伤的保护机制。方法血压正常的SD孕鼠(妊娠第14天)30只,随机取6只作为对照组(等体积生理盐水灌胃),其余24只孕鼠采用亚硝基左旋精氨酸甲酯诱导法建立妊娠期高血压大鼠模型;将造模成功的24只孕鼠随机分为模型组(等体积生理盐水灌胃)、维生素D低、高剂量组(维生素D 0.03μg/kg、0.15μg/kg灌胃)、卡托普利组(卡托普利30 mg/kg灌胃),每组6只;治疗后第7天测量大鼠尾动脉收缩压、舒张压;肝功能检测血清丙氨酸转氨酶(ΑLT)和天冬氨酸氨转氨酶(ΑST);油红染色评估肝组织病理变化;western blotting法检测肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1β蛋白的表达。结果与模型组比较,维生素D低剂量组、维生素D高剂量组以及卡托普利组其舒张压、收缩压、平均动脉压明显下降,具有显著统计学意义(P<0.05);与模型组比较,维生素D低剂量组、维生素D高剂量组以及卡托普利组ΑLT、ΑST水平均明显降低(P<0.05);形态学观察发现,与模型组相比,维生素D低剂量组、高剂量组以及卡托普利组肝损伤减轻;Western blotting结果显示,维生素D低、高剂量组及卡托普利组TNF-α、IL-1β表达较模型组显著降低(P<0.05)。结论维生素D可以下调TNF-α,IL-1β蛋白表达,抑制肝脏炎症,降低妊娠期高血压大鼠血压,保护肝脏。

Objective To observe the effect of Vitamin D on the liver in hypertensive rats and investigate the protection mechanism of Vitamin D on liver injury in pregnant hypertensive rats.Methods 30 SD pregnant rats with normal blood pressure were selected.6 of them were randomly selected as control group(equal volume normal saline gavage),and 24 rats in the model group(The rat pregnancy-induced hypertension syndrome model induced by methyl ester was established).After the PIH model established successfully,the 24 rats were equally divided into four groups[model group(equal volume normal saline gavage),low dose of Vitamin D group(50 IU/g gavage),high dose of Vitamin D(100 IU/g gavage),captopril group(30 mg/kg gavage)].Seven days after treatment,the tail artery systolic pressure of rats was measured.After 5 weeks,Oil red O staining was used to observe morphological changes and evaluate the pathological changes of the liver,liver function test serum alanine aminotransferase(ΑLT)and aspartate aminotransferase(ΑST).Western blotting was used to measure the expression of TNF-αand IL-1β.Results Compared with the model group,the diastolic blood pressure,systolic blood pressure and mean arterial blood pressure in low dose of Vitamin D group,high dose of Vitamin D and captopril group had a significant decrease,which had statistical significance(P<0.05).The levels of ALT and AST were significantly lower than those in the control group(P<0.05).Morphological observation showed liver injury in the control group.Compared with the model group,low dose and high dose of Vitamin D and captopril group could alleviate the damage of liver.Western blotting showed a significant decrease of TNF-αand IL-1βin low dose of Vitamin D group,high dose of Vitamin D and captopril group.Conclusion Vitamin D can down-regulate the expression of TNF-αand IL-1β,inhibit liver inflammation,reduce blood pressure in hypertensive rats during pregnancy and protect the liver.