UPLC法同时检测青蒿废弃药渣发酵物中青蒿素、双氢青蒿素、青蒿琥酯含量

Simultaneous determination of artemisinin,dihydroartemisinin and artesunate inartemisia annua waste leaven by UPLC

以青蒿废弃药渣发酵物为实验材料,利用UPLC法同时测定发酵物中青蒿素、双氢青蒿素、青蒿琥酯3个成分的含量。采用岛津Shim-pack XR-ODS II色谱柱(75 mm×2.0 mm i.d.,2.2μm),以0.1%醋酸溶液:乙腈(50∶50)为流动相,流速0.35 mL/min,检测波长195 nm,柱温30℃,进样量3μL进行检测。结果表明,青蒿药渣发酵物中含有青蒿素、双氢青蒿素、青蒿琥酯3个成分且在15 min内能够完全分离,3个成分分别在5.02~1004,1.315~263,2.59~518μg/mL范围内线性关系良好,平均加样回收率为分别为99.34%,98.87%,99.46%,RSD为1.91%,1.52%,1.67%,3批药渣发酵物中青蒿素、双氢青蒿素、青蒿琥酯的含量范围分别为543.8~689.3,98.7~152.9,468.7~502.1μg/g。该研究表明利用UPLC法可同时检测青蒿药渣发酵物中青蒿素、双氢青蒿素、青蒿琥酯3个有效成分,为中药材资源再利用创造条件,给青蒿药材及其相关产品的质量控制提供技术支持。

Using artemisia annua waste leaven as the experimental material,the contents of artemisinin,dihydroartemisinin and artesunate were determined simultaneously by UPLC method.The determination was performed on a Shimadzu Shim-pack XR-ODS II column(75 mm×2.0 mm i.d.,2.2μm)with the mobile phase consisting of 0.1%acetic acid solution-acetonitrile(50∶50)at a flow rate of 0.35 mL/min.The column temperature was 30℃and the detection wavelength was 195 nm and the injection volume was 3μL.The results showed that artemisinin,dihydroartemisinin and artesunate were contained in artemisia annua waste leaven and could be completely separated within 15 min.The linear ranges of artemisinin,dihydroartemisinin and artesunate were 5.02~1004,1.315~263,2.59~518μg/mL,respectively.The recoveries of the above-mentioned components were 99.34%,98.87%,99.46%,as well as the relative standard deviations were 1.91%,1.52%,1.67%,respectively.The content ranges of artemisinin,dihydroartemisinin and artesunate in 3 batches of leaven of medicinal residue were 543.8~689.3,98.7~152.9,468.7~502.1μg/g,respectively.This study shows that the effective components of artemisinin,dihydroartemisinin and artesunate in artemisia annua leaven can be detected simultaneously by UPLC method,creating conditions for the reuse of Chinese medicinal materials and providing technical support for the quality control of artemisia annua and its related products.