2019年四川省风疹毒株分子流行病学特征分析

Molecular epidemiological characteristics of rubella virus isolates in Sichuan Province in 2019

目的分析四川省风疹病毒(rubella virus,RV)基因特征。方法采用Vero/SLAM细胞对2019年四川省疑似风疹病例咽拭子标本进行风疹病毒分离培养,通过逆转录-聚合酶链(reverse transcription-polymerase chain reaction,RTPCR)反应扩增编码风疹病毒E1基因的739个核苷酸片段,并对扩增产物进行序列测定和同源性分析。结果2019年四川省1372份疑似风疹病例咽拭子标本共分离到873株风疹病毒,获得865份毒株的有效序列,其中729株为2B基因型,136株为1E基因型,与四川省以前的风疹病毒分离株在不同的小分支上。2B基因型分离株之间的核苷酸和氨基酸同源性分别为95.6%~97.6%和87.6%~93.8%;1E基因型分离株之间的核苷酸和氨基酸同源性分别为97.4%~98.2%和92.1%~95.0%。结论2019年四川省风疹病例由输入型2B基因型和1E基因型风疹病毒引起。

Objective To analyze the genetic characteristics of rubella virus(RV)in Sichuan Province.Methods Vero/SLAM cells were used to isolate and culture rubella virus from throat samples of suspected rubella cases in Sichuan Province in 2019.The 739 nucleotides encoding the rubella virus E1 gene were amplified by Reverse Transcription-Polymerase Chain Reaction(RTPCR)and the amplified products were sequenced and homology analyzed.Results A total of 873 strains of rubella virus were isolated from 1372 throat swab samples of suspected rubella cases in Sichuan Province in 2019.A total of 865 effective sequences were obtained,including 729 strains of genotype 2B and 136 strains of genotype 1E,which were located on a different subbranch from the previous strains of rubella virus in Sichuan Province.The nucleotide and amino acid homologies of genotype 2B isolates were 95.6%-97.6%and 87.6%-93.8%,respectively.The nucleotide and amino acid homologies of genotype 1E isolates were 97.4%-98.2%and 92.1%-95.0%,respectively.Conclusions Rubella cases in Sichuan Province in 2019 were caused by imported rubella virus genotype 2B and 1E.