单细胞荟萃分析SARS-CoV-2感染后T淋巴细胞在各个器官中的功能差异

Single-cell meta-analysis of T lymphocytes functional differences in various organs after SARS-CoV-2 infection

目的了解SARS-CoV-2感染后T淋巴细胞在各个器官中的功能差异。方法应用生物信息学方法,利用高通量基因表达数据库数据(GSE171668、GSE159812、GSE159556、GSE167747)及单细胞技术的分析方法,分析SARS-CoV-2感染患者各器官的T淋巴细胞在体内的功能差异。结果通过对新冠肺炎患者16个肝脏、19个心脏、2个脾脏、6个大脑、58个肺、21个肾脏、5个胰腺组织的单细胞数据提取,发现入侵基因在肺和胰腺的T淋巴细胞中表达相对较高。肺的T淋巴细胞高表达干扰素信号通路,而其他器官表达相对较低;同时肺的T淋巴细胞也高表达脂肪酸结合位点。结论SARS-CoV-2感染后,相对于其他器官,肺的T淋巴细胞具有特殊的干扰素活化信号通路和脂肪酸结合位点。

Objective To explore the functional heterogeneity of T lymphocytes in various organs after SARSCoV-2 infection. Methods Using the public database GEO data(GSE171668, GSE159812, GSE159556, GSE167747) and the analysis method of single-cell technology, the functional differences of T lymphocytes in various organs of patients after infection with SARS-CoV-2 were analyzed. Results Through single-cell data extraction of 16 livers, 19 hearts,2 spleens, 6 brains, 58 lungs, 21 kidneys and 5 pancreases from SARS-CoV-2 infected patients, invasion genes were relatively highly expressed in T lymphocytes of the lung and pancreas. The lung had a special ability to express the interferon signaling pathway, while the expression of other organs was relatively low;at the same time, the T lymphocytes of the lung also highly expressed fatty acid binding sites. Conclusion After SARS-CoV-2 infection, compared with other organs, the lung has a special interferon-activated signaling pathway and fatty acid binding site.