新型生物催化剂催化合成植物甾醇酯工艺优化

Process Optimization for the Synthesis of Phytosterol Esters Catalyzed by A Novel Biocatalyst

以具有3D开放孔隙特征的介孔有机硅球为载体构建新型生物催化剂,并用于催化合成植物甾醇酯。采用单因素试验,考察底物类型、底物浓度、酶用量、温度和反应时间5个因素对植物甾醇转化率的影响。通过响应面分析法确定植物甾醇酯的最佳合成条件,当棕榈酸(27.5 mmol/L)作为底物、酶用量21.4 U、反应温度47.2℃、反应时间11.4 h时,植物甾醇获得最大转化率为(96.8±0.7)%。此外,催化剂重复使用10次后,甾醇转化率为97.11%,表明该催化剂具有良好的稳定性。

A novel biocatalyst was constructed with mesoporous organosilica spheres(with 3D open channels)as support and used for the synthesis of phytosterol esters. The effects of substrate type,substrate concentration,enzyme dosage,reaction temperature,and reaction time on the conversion of phytosterol were investigated by single factor tests. The synthesis conditions of phytosterol esters were optimized by response surface methodology. The highest conversion of phytosterols was(96.8±0.7)% in the case of 27.5 mmol/L palmitic acid as substrate,enzyme dosage of 21.4 U,the reaction temperature at 47.2 ℃ and the reaction time of 11.4 h. In addition,the sterol conversion rate was 97.11% after the biocatalyst was reused for 10 times,which demonstrated that the biocatalyst owned good stability.