3D回旋仪模拟微重力效应对小鼠骨骼和心脏的影响

3D Clinostat Simulates the Effects of Microgravity on the Bones and Hearts of Mice

目的:利用3D回旋仪模拟微重力效应探究对小鼠骨骼和心脏的影响。方法:25只8周龄雄性C57BL/6J小鼠,随机分成5组(n=3~5):地面对照组(GC组),3周处理对照组(TC3组),3周模拟微重力组(SM3组),6周处理对照组(TC6组),6周模拟微重力组(SM6组)。使用Micro-CT检测小鼠左股骨参数变化。采用超声方法检测小鼠心脏结构和功能的变化。结果:与GC组相比,TC3组、SM3组、TC6和SM6组小鼠的骨体积分数,骨小梁厚度显著降低(P<0.05),骨小梁间距显著增加(P<0.05)。骨小梁数量和皮质骨壁厚度没有显著性差异。3D回旋仪处理3周后,与小鼠左心室后壁厚度变薄相比,其他参数没有变化。而在第6周,舒张期和收缩期的左心室内径变小,左心室质量变轻。结论:3D回旋仪模拟微重力效应会导致小鼠骨质流失,影响心脏结构和功能,且具有时间效应。

Objective:To explore the effect of 3D clinostat simulation microgravity effect on mice bones and heart.Methods:Twenty-five 8-week-old male C57BL/6J mice were randomly divided into 5 groups(n=3~5):ground control group(GC group),3-week-treated control group(TC3 group),3 weeks simulated microgravity group(SM3 group),6-week-treated control group(TC6 group),6 weeks simulated microgravity group(SM6 group).Micro-CT was used to detect the changes of left femur parameters in mice.Results:The results showed that compared with GC group,the bone volume/tissue volume and trabecular thickness of mice in TC3,SM3,TC6 and SM6 groups were significantly decreased(P<0.05),the trabecular spaceing was significantly increased(P<0.05).There was no significant difference in trabecular number and cortical bone wall thickness.After 3 weeks of treatment with the 3D clinostat,other heart parameters did not change compared to the thinning of the LVPW in mice.Diastolic and systolic left ventricle diameter decreased,LV Mass became lighter,and tight space led to increased stroke volume and ejection fraction.Conclusion:Microgravity effects simulated by 3D clinostat can lead to bone loss in mice,and affect the structure and function of the heart with time effect.