壳聚糖接枝的还原氧化石墨烯在聚磷酸钙多孔支架上的构建

Construction of Chitosan-Grafted Reduced Graphene Oxide on Calcium Polyphosphate Porous Scaffold

通过酰胺反应制备了壳聚糖接枝的还原氧化石墨烯(CS-rGO),并首次将其作为自组装涂层应用于聚磷酸钙(CPP)多孔支架的表面改性。红外光谱、X射线光电子能谱、X射线衍射和拉曼光谱等表征证明了CS-rGO的成功制备;形态学观察发现,CS-rGO在CPP多孔支架表面上形成了均匀稳定的涂层;力学测试结果表明,CS-rGO涂层增强了CPP多孔支架的压缩强度;体外矿化实验表明,CS-rGO涂层提高了CPP多孔支架的矿化能力;抗菌实验表明,在与大肠杆菌和金黄色葡萄球菌共培养24 h之后,CPP/CS-rGO/0.3多孔支架对大肠杆菌和金黄色葡萄球菌的抗菌率分别为66%和71%。

Chitosan-grafted reduced graphene oxide(CS-rGO)was successfully prepared via amide reaction.Then,CS-rGO was first used as self-assembled coating for the surface modification of calcium polyphosphate(CPP)porous scaffolds.CS-rGO was characterized by FT-IR,XPS,XRD and Raman;the morphological observation indicates that CS-rGO is uniformly and stably distributed on the surface of CPP porous scaffold;the results of the mechanical experiment shows that CS-rGO can improve the compressive strength of CPP porous scaffolds;the in vitro mineralization experiment show that CS-rGO can promote the mineralization ability of CPP porous scaffolds;the antibacterial experiment indicates that after 24 h exposure of scaffold to bacteria,about 71%of S.aureus and 66%of E.coli are inhibited in the CPP/CS-rGO/0.3 scaffold.