miR-107靶向肿瘤蛋白D52抑制前列腺癌细胞的增殖和侵袭

miR-107 suppresses cell proliferation and invasion by targeting tumor protein D52 in prostate cancer

目的:研究microRNA-107(miR-107)对前列腺癌细胞生物学功能的影响和潜在的作用机制。方法:采用瞬时转染、细胞克隆、细胞划痕、侵袭实验检测miR-107组与对照组(miR-NC组)对前列腺癌细胞生物学功能的影响。此外,通过靶基因预测软件TargetscanHuman7.2筛选出肿瘤蛋白D52(TPD52)作为miR-107的潜在下游靶基因并采用双荧光素酶报告基因实验进行了特异性结合的验证。同时,进一步通过蛋白免疫印迹和免疫荧光实验进行靶基因的表达检测。结果:miR-107组的细胞划痕愈合率、侵袭率、增殖率均低于miR-NC组(P<0.01)。miR-107组对靶基因TPD52表达量小于miR-NC组(P<0.01)。结论:miR-107通过靶向TPD52抑制前列腺癌的增殖和侵袭。

Objective:To investigate the effects and potential mechanism of miR-107 in prostate cancer.Methods:Transient transfection,cell cloning,cell scratching and invasion experiments were used to detect the effects of miR-107 group and control group(miR-NC) on the biological functions of prostate cancer cells.In addition,we selected tumor protein D52(TPD52) as a potential downstream target gene of miR-107 by TargetscanHuman7.2,and used dual-luciferase reporter assay for verifing the specific binding.Meanwhile,Western blotting and immunofluorescence assays were performed to detected the expression of target gene.Results:The healing rate,invasion rate and proliferation rate of cell scratch in miR-107 group were lower than those in miR-NC group(P<0.01).The expression of target gene TPD52 in miR-107 group was less than that in miR-NC group(P<0.01).Conclusions:miR-107 inhibits the proliferation and invasion of prostate cancer by targeting TPD52.