上海市虹口区HIV-1感染者分子流行病学研究

Molecular epidemiological study on HIV-1 patients in Hongkou District,Shanghai

【目的】分析上海市虹口区新确认未治疗人类免疫缺陷病毒(HIV)⁃1感染者的HIV⁃1亚型分布特征,为虹口区建立HIV⁃1亚型数据库,探索获得性免疫缺陷综合征(AIDS)精准防治策略提供基础数据和思路。【方法】虹口区2016年新确认的HIV⁃1感染者的血浆样品119份,对HIV⁃1 RNA pol区基因进行反转录和基因扩增,扩增后进行DNA测序,在Los Alamos2022 HIV⁃1基因数据库网站上使用HIV⁃BLAST工具进行HIV⁃1亚型分析,并结合流行病学资料进行分析。【结果】119份样品中,共获得89例pol区基因片段,以CRF07_BC(38.2%)、CRF01_AE(36.0%)为主,B亚型占13.5%,并发现新型重组亚型CRF55_01B和独特重组亚型URF_0107各2例(2.3%)。【结论】2016年,虹口区HIV⁃1感染者HIV⁃1流行亚型以CRF07_BC和CRF01_AE为主,新的重组亚型CRF55_01B和独特重组亚型URF_0107已在虹口区出现,密切监视其流行趋势十分必要。

[Objective]To determine the characteristics of human immunodeficiency virus type 1(HIV-1)subtypes among newly confirmed HIV-1 patients in Hongkou District of Shanghai,and provide evidence for establishing HIV-1 subtype database and precise prevention and control strategy against AIDS in Hongkou District of Shanghai.[Methods]A total of 119 plasma samples from newly confirmed HIV-1 patients in Hongkou District in 2016 were used to detect HIV-1 RNA pol gene by using reverse transcription PCR.HIV-1 subtypes were determined with Los Alamos 2022 HIV-1 gene database using HIV-BLAST tool,and then characterized by epidemiological data.[Results]In the 119 samples,a total of 89 pol gene fragments were obtained,which were mainly classified as CRF07_BC(38.2%),CRF01_AE(36.0%),and subtype B(13.5%).Furthermore,two new recombinant subtype CRF55_01B and two unique recombinant subtype URF_0107 were identified(2.3%).[Conclusion]In 2016,HIV-1 subtypes in Hongkou District are mainly CRF07_BC and CRF01_AE.In addition,new recombinant subtype CRF55_01B and unique recombinant subtype URF_0107 have appeared in Hongkou District.It warrants a need of enhanced surveillance.