摘要
目的 探讨核因子-κB(NF-κB)抑制剂四氢化吡咯二硫代氨基甲酸酯(PDTC)对胶质瘤干细胞放射治疗增敏的效用。方法 用无血清悬浮培养法培养胶质瘤干细胞,培养后,将细胞分成C组(加二甲基亚砜30μmol/L)、PDTC组(30μmol/L,加PDTC)、放疗组(8 Gy,加二甲基亚砜30μmol/L)和PDTC联合放疗组。采用Western blot比较各组放疗后36 h、48 h、72h胶质瘤干细胞中NF-κB蛋白表达及细胞凋亡情况。结果 X线照射后,胶质瘤干细胞中NF-κB蛋白表达明显增加,与C组比较差异有显著性(P<0.05),而PDTC可下调NF-κB蛋白表达,差异有统计学意义(P<0.05)。PDTC组、放疗组和联合组的胶质瘤干细胞凋亡率与C组比较,依次半圆(1.50±0.16)倍、(2.48±0.15)倍、(6.55±0.24)倍。结论 NF-κB通路在胶质瘤干细胞的放射治疗中起关键作用,并可以作为对胶质瘤干细胞放疗增加敏感性的重要靶点。
Objective To explore nuclear factor-κB(NF-κB)Effect of inhibitor tetrahydropyrrole dithiocarbamate(PDTC)on radiosensitization of glioma stem cells.Methods Glioma stem cells were cultured by serum-free suspension culture.After culture,the cells were divided into group C(30 μmol/L dimethyl sulfoxide),PDTC group(30 μmol/L),radiotherapy group(8 Gy,30 μmol/L dimethyl sulfoxide)and PDTC combined radiotherapy group.NF in glioma stem cells at 36 h,48 h and 72 h after radiotherapy were compared by Western blot NF-κB protein expression and apoptosis.Results After X-ray irradiation,The expression of NF-κB protein increased significantly compared with group C(P<0.05),and PDTC could down regulate NF-κB There was significant difference in the expression of NF-κB protein(P<0.05).Compared with group C,the apoptosis rates of glioma stem cells in PDTC group,radiotherapy group and combined group were semicircular(1.50±0.16),(2.48±0.15)and(6.55±0.24)times.Conclusion NF-κB pathway plays an important role in the radiotherapy of glioma stem cells,and can be used as an important target to increase the sensitivity of glioma stem cells to radiotherapy.
作者
邓庆芬
刘晓丽
周玉璞
DENG Qingfen;LIU Xiaoli;ZHOU Yupu(Henan Second People's Hospital,Xinzheng,451191)
出处
《实用癌症杂志》
2022年第10期1575-1577,共3页
The Practical Journal of Cancer
关键词
核因子
胶质瘤干细胞
放疗
增敏
Nuclear factor
Glioma stem cells
Radiotherapy
Sensitization